超临界CO<sub>2</sub>流体萃取苦丁茶多糖的工艺优化

韦晓洁<sup>1; 2*</sup>; 银慧慧<sup>1; 2</sup>; 孟 菲<sup>1; 2</sup>; 赵 武<sup>1; 2</sup>; 刘 伟<sup>1; 2</sup>; 姜源明<sup>1; 2</sup>; 覃振华<sup>1; 2</sup>; 孙建华<sup>1; 2</sup>; 陈海兰<sup>3</sup>

引用本文:	韦晓洁, 银慧慧, 孟菲, 赵武, 刘伟, 姜源明, 覃振华, 孙建华, 陈海兰.超临界CO₂流体萃取苦丁茶多糖的工艺优化[J].广西植物,2018,38(5):590-595.[点击复制]
	WEI Xiaojie, YIN Huihui, MENG Fei, ZHAO Wu, LIU Wei, JIANG Yuanming, QIN Zhenhua, SUN Jianhua, CHEN Hailan.Supercritical CO₂ fluid extraction process optimization for polysaccharides from Ilex kudingcha[J].Guihaia,2018,38(5):590-595.[点击复制]

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超临界CO₂流体萃取苦丁茶多糖的工艺优化
韦晓洁^1,2*, 银慧慧^1,2, 孟菲^1,2, 赵武^1,2, 刘伟^1,2, 姜源明^1,2, 覃振华^1,2, 孙建华^1,2, 陈海兰³
1. 广西壮族自治区兽医研究所, 南宁 530001;2. 广西兽医生物技术重点实验室, 南宁 530001;3. 广西大学动物科学技术学院, 南宁 530001

摘要:

为了探讨超临界CO₂萃取广西苦丁茶中多糖的工艺条件,该研究采用超临界CO₂流体萃取技术分离苦丁茶多糖,利用苯酚-硫酸法对苦丁茶多糖含量进行测定,并考察不同萃取温度(35、40、45、50、55、60 ℃)、萃取压力(20、25、30、35、40、45、50 MPa)、萃取时间(30、60、90、120、150 min)、夹带剂(甲醇、95%甲醇、50%甲醇、无水乙醇、95%乙醇、50%乙醇)以及夹带剂(95%乙醇)用量(2.0、2.5、3、3.5、4.0、4.5、5.0 mL·min^-1)对多糖得率的影响,通过设计正交实验方案,对超临界CO₂萃取广西苦丁茶多糖的提取工艺进行优化。结果表明:通过单因素和正交实验考察了苦丁茶多糖提取的主要影响因素,得到的最佳萃取工艺条件为萃取温度50 ℃,萃取压力40 MPa,夹带剂流量3.5 mL·min^-1,萃取时间150 min; 采用苯酚-硫酸法对苦丁茶多糖含量进行测定。在最优萃取条件下得到的苦丁茶多糖的提取率为7.05%。由此可知,采用超临界CO₂流体萃取,具有提取温度低、萃取率高、萃取周期短、低耗以及污染小等优点,适用于苦丁茶多糖的提取。

关键词: 超临界CO₂, 苦丁茶, 多糖, 单因素, 正交实验

DOI：10.11931/guihaia.gxzw201706021

分类号:Q946.889

文章编号:1000-3142(2018)05-0590-06

基金项目:中国博士后科学基金(2017M613271XB); 广西畜禽疫苗新技术重点实验室开放基金(15-140-31-B-2)[Supported by the Postdoctoral Science Foundation of China(2017M613271XB); Open Foundation of Guangxi Key Laboratory of Animal Vaccines and New Technology(15-140-31-B-2)]。

Supercritical CO₂ fluid extraction process optimization for polysaccharides from Ilex kudingcha

WEI Xiaojie^1,2*, YIN Huihui^1,2, MENG Fei^1,2, ZHAO Wu^1,2, LIU Wei^1,2, JIANG Yuanming^1,2, QIN Zhenhua^1,2, SUN Jianhua^1,2, CHEN Hailan³

1. Guangxi Veterinary Research Institute, Nanning 530001, China;2. Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostic, Nanning 530000, China;3. Institute of Animal Science and Technology of Guangxi University, Nanning 530001, China

Abstract:

In order to discuss the process conditions for extracting polysaccharides from Ilex kudingcha by supercritical CO₂ fluid, the polysaccharides from I. kudingcha were extracted and analyzed by supercritical CO₂ fluid extraction. Phenol-sulfuric acid method was applied for the determination of polysaccharides. Effects of different extraction temperatures(35, 40, 45, 50, 55, 60 ℃), extraction pressures(20, 25, 30, 35, 40, 45, 50 MPa), extraction time(30, 60, 90, 120, 150 min), cosolvents(methanol, 95% methanol, 50% methanol, ethanol, 95% ethanol, 50% ethanol)and cosolvent flow rates(95% ethanol)(2.0, 2.5, 3, 3.5, 4.0, 4.5, 5.0 mL·min^-1)on the polysaccharides extraction rate were investigated and the process conditions for extracting polysaccharides from I. kudingcha by supercritical CO₂ fluid were optimized through orthogonal experiments. Single factor and orthogonal experiments were used to investigate the factors on the yield of polysaccharides. The optimal operation conditions included extraction temperature 50 ℃, extraction pressure 40 MPa, extraction time 150 min, cosolvent flow rate 3.5 mL·min^-1. The concentration of polysaccharides was determined by phenol-sulfuric acid method and the extraction rate was 7.05% under the optimal extraction conditions. The results showed that supercritical CO₂ fluid extraction technology was favorable for extracting polysaccharides from I. kudingcha with the advantages of low extraction temperature, high extraction rate, short extraction period, the environment-friendly and energy-saving.

Key words: supercritical CO₂ fluid, Ilex kudingcha, polysaccharides, single factor, orthogonal experiments