| 引用本文: | 蒋谦才, 谭宗健, 廖浩斌, 刘盼盼, 孙红梅, 王瑞江,
曹洪麟, 黄向旭, 赖思茹, 梁 丹, 王峥峰.广东省中山市五桂山土沉香遗传多样性[J].广西植物,2018,38(6):804-811.[点击复制] |
| JIANG Qiancai, TAN Zongjian, LIAO Haobin, LIU Panpan, SUN Hongmei,
WANG Ruijiang, CAO Honglin, HUANG Xiangxu, Lai Siru,
Liang Dan, WANG Zhengfeng.Genetic diversity of Aquilaria sinensis in Wuguishan, Zhongshan, Guangdong Province[J].Guihaia,2018,38(6):804-811.[点击复制] |
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| 广东省中山市五桂山土沉香遗传多样性 |
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蒋谦才1, 谭宗健1, 廖浩斌1, 刘盼盼1, 孙红梅1, 王瑞江2,
曹洪麟2, 黄向旭2, 赖思茹2, 梁 丹2, 王峥峰2*
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1. 中山市国有森林资源保护中心, 广东 中山 528400;2. 中国科学院华南植物园, 广州 510650
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| 摘要: |
| 土沉香(Aquilaria sinensis)是生产中药沉香的重要树种,现被列为国家二级重点保护野生植物。该研究通过采集该地区83株土沉香样品,利用限制性酶切位点相关DNA测序(restriction site-associated DNA sequencing,RAD-seq)方法获得的18个微卫星体作为分子标记,计算了五桂山土沉香种群的整体观测杂合度(observed-heterozygosity)、期望杂合度(expected-heterozygosity)、种群整体近交系数及其个体间的亲缘关系,并对种群可能经历的种群缩小或种群扩张状况进行了分析。结果表明:与同属其它物种以及同一生活史物种相比,五桂山地区土沉香遗传多样性略低(观测杂合度和期望杂合度分别为0.523和0.522),但种群整体处于随机交配状态(近交系数为-0.002)。瓶颈效应分析表明该种群没有经历瓶颈效应,且81.810%的个体间无亲缘关系,说明这一地区土沉香并非少数个体发育起来; 种群扩张分析表明其种群大小有增长情形。综合这些结果可以看出,中山市五桂山土沉香种群整体遗传健康状况良好,较好地保存了该区域种质资源遗传多样性,为今后的保护和合理开发提供了优质资源储备 |
| 关键词: 瓶颈效应, 亲缘关系, 微卫星体, 遗传变异, 种群扩张 |
| DOI：10.11931/guihaia.gxzw201709027 |
| 分类号:Q319+.1 |
| 文章编号:1000-3142(2018)06-0804-08 |
| 基金项目:广东省科技计划项目(2017A030303060); 中山市财政局资金项目,东莞市科技项目(2015108101002); 广东省林业厅中药材保护项目(2017); 广东省林业厅野生动植物保护和湿地保护专项项目(2130299); 广东省野生动植物保护管理项目(2016)[Supported by Science and Technology Program of Guangdong Province(2017A030303060); Zhongshan Finance Bureau, Science and Technology Program of Dongguan City(2015108101002); Conservation of Traditional Chinese Medicine by Forestry Administration of Guangdong(2017); Special Foundation for Conservation of Wild Animals, Plants and Wetlands by Forestry Administration of Guangdong(2130299); Program for Conservation and Management of Wild Animals and Plants in Guangdong Province(2016)]。 |
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| Genetic diversity of Aquilaria sinensis in Wuguishan, Zhongshan, Guangdong Province |
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JIANG Qiancai1, TAN Zongjian1, LIAO Haobin1, LIU Panpan1, SUN Hongmei1,
WANG Ruijiang2, CAO Honglin2, HUANG Xiangxu2, Lai Siru2,
Liang Dan2, WANG Zhengfeng2*
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1. Zhongshan State-owned Forest Resources Protected Center, Zhongshan 528407, Guangdong, China;2. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
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| Abstract: |
| Aquilaria sinensis, famous of its fragrant wood, is listed as national Level Ⅱ protected wild plants. For sound conservation this precious population of A. sinensis in Wuguishan District of Zhongshan City, we conducted genetic diversity surveys on it. Because population history such as shrinking or expansion would definitely leave signatures in population genetic variations, based on the genetic diversity investigated in Wuguishan population of A. sinensis, we also made population history inferences for it. Among genetic diversity studies, microsatellite markers are the most suitable tools due to their high polymorphism. Therefore, using eighteen microsatellites obtained by restriction site-associated DNA sequencing(RAD-seq), we examined the genetic diversities of 83 A. sinensis individuals collected from Wuguishan District, and estimated genetic diversity parameters by observed-heterozygosity, expected-heterozygosity and inbreeding index. Because the genetic relationship between individuals could reflect whether Wuguishan population of A. sinensis was derived from a few ancestors, pairwise genetic relatedness was calculated and four types of relationship(parent-offspring, full-siblings, half-siblings and unrelated)between individuals were determined. The population history was inferred for population shrinking(bottleneck)or expansion. Overall, compared to the genetic variation of the species in the same genus and the species with similar life history traits, the genetic diversity in Wuguishan population was slightly low with the observed-heterozygosity and expected-heterozygosity being 0.523 and 0.522, respectively. The value of -0.002 for inbreeding index, which was close to zero, indicated that the whole population was under random mating. Bottleneck analysis indicated bottleneck effects did not happened recently in Wuguishan population, which meant no population shrinking. Pairwise relationships between individuals confirmed this by showing most of individual pairs(>81.810%)were unrelated. Finally, we detected the evidence of population expansion on the basis of allele size distributions. Accordingly, these results indicated that Wuguishan population of A. sinensis is generally genetically healthy. Such information provides the reference for the conservation and the reasonable uses of A. sinensis in the future. |
| Key words: bottleneck effects, genetic relationship, microsatellites, genetic variation, population expansion |
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