彩色大白菜子叶的不定芽再生

屈会玲<sup>1; 2</sup>; 张鲁刚<sup>1*</sup>; 范爱丽<sup>1</sup>

引用本文:	屈会玲, 张鲁刚, 范爱丽.彩色大白菜子叶的不定芽再生[J].广西植物,2007,(5):748-754.[点击复制]
	QU Hui-Ling, ZHANG Lu-Gang, FAN Ai-Li.Shoot regeneration from cotyledon of orange-heading Chinese Cabbage[J].Guihaia,2007,(5):748-754.[点击复制]

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彩色大白菜子叶的不定芽再生
屈会玲^1,2, 张鲁刚^1*, 范爱丽¹
1.西北农林科技大学园艺学院, 陕西杨凌 712100;2.山西省运城市农业局, 山西运城 044000

摘要:

以彩色大白菜子叶为外植体,研究不同激素配比和AgNO₃对不定芽再生的影响。结果表明:单独附加细胞分裂素(6-BA或TDZ)的MS培养基,不能诱导子叶不定芽分化; 而同时附加生长素(NAA)和细胞分裂素(6-BA或TDZ),不定芽的再生频率提高,最高为15%; AgNO₃与细胞分裂素及生长素配合使用,能大幅度提高子叶不定芽的再生频率,提高率最高达42.5%。与6-BA相比,TDZ对不定芽再生的效果更好。当TDZ浓度为0.05 mg/L、NAA为0.3 mg/L、AgNO₃为8 mg/L时,产生丛状芽数目最多,再生率最高,达50%。

关键词: 彩色大白菜子叶片再生

DOI：

分类号:Q943.1

基金项目:

Shoot regeneration from cotyledon of orange-heading Chinese Cabbage

QU Hui-Ling^1,2, ZHANG Lu-Gang^1*, FAN Ai-Li¹

1.College of Horticulture, Northwest Agriculture and Forestry University, Yangling 712100, China;2.Agricultural Bureau of Yuncheng City, Yuncheng 044000, China

Abstract:

The effects of hormone combination and AgNO₃ on adventitious shoot regeneration from cotyledon of orange-heading Chinese Cabbage were studied. The results showed that the adventitious shoot could not be induced from the cotyledon in MS medium added with cell division hormone(6-BA or TDZ)only; the regeneration frequency of shoot reached to 15% in MS medium added with cell division hormone(6-BA or TDZ)and growth hormone(NAA)together; the regeneration frequency could increase 42.5% in MS medium added with cell division hormone(TDZ),growth hormone(NAA)and AgNO₃. Compared with 6-BA,TDZ had more effective in inducing shoot regeneration. The maximum frequency of shoot regeneration(50%)was obtained in MS medium which contained 0.05 mg/L TDZ,0.3 mg/L NAA and 8 mg/L AgNO₃.

Key words: Brassica campestris ssp.pekinensis cotyledon regeneration