| 摘要: |
| 建立了红丝线提取物中紫蓝素的测定方法。采用反相高效液相色谱法,色谱柱为ZORBAX XDB-C18(4.6 mm×150 mm,5 μm); 流动相:V(乙腈):V[75 mmol/L乙酸铵+0.5 mmol/L EGTA(pH7.0)]=8:92; 流速:1 mL/min; 检测波长590 nm。紫蓝素的线性范围为2.5～50 mg/L(r=0.999 9),回收率97.9%～101.5%。该法简便、准确,重复性好,适用于测定红丝线提取物中紫蓝素的含量。 |
| 关键词: 高效液相色谱法 紫蓝素 红丝线提取物 |
| DOI： |
| 分类号:Q946 |
| 基金项目: |
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| Analysis of zilanine in extract of Peristrophe baphica by RP-HPLC |
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JIANG Xiao-Hua, XIE Yun-Chang*, HUANG Yong-Lin
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Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China
Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China
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| Abstract: |
| The paper established a RP-HPLC method for the determination of the content of zilanine in extract of Peristrophe baphica. RP-HPLC was conducted on a ZORBAX XDB-C18(4.6 mm×150 mm,5 μm)with CH3CN-[CH3COONH4(75 mmol/L)+EGTA(0.5 mmol/L,pH=7.0)]=8:92; flow rate 1.0 mL/min,the detection wavelength was set at 590 nm. The linear range of zilanine was 2.5～50 mg/L(r=0.9999). The recoveries were 97.9%～101.5%. The method is simple and accurate with good reproducibility,it is suitable for the determination of zilanine content in extract of P.baphica. |
| Key words: HPLC zilanine extract of Peristrophe baphica |
