辣椒CaNPR1-RNAi表达载体的
构建及其对辣椒的转化

罗 欢<sup>1; 2</sup>; 段承杰<sup>1; 2</sup>; 陈保善<sup>1; 2</sup>; 唐纪良<sup>1; 2</sup>; 冯家勋<sup>1; 2*</sup>

引用本文:	罗欢, 段承杰, 陈保善, 唐纪良, 冯家勋.辣椒CaNPR1-RNAi表达载体的构建及其对辣椒的转化[J].广西植物,2008,(1):107-112.[点击复制]
	LUO Huan, DUAN Cheng-Jie, CHEN Bao-Shan TANG Ji-Liang, FENG Jia-Xun.Construction of pepper CaNPR1-RNAi expression vector and its transformation to pepper[J].Guihaia,2008,(1):107-112.[点击复制]

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*辣椒CaNPR1-RNAi表达载体的构建及其对辣椒的转化*
罗欢^1,2, 段承杰^1,2, 陈保善^1,2, 唐纪良^1,2, 冯家勋^1,2*
1.广西亚热带生物资源保护利用重点实验室, 南宁 530005;2.广西大学生命科学与技术学院, 南宁 530005

摘要:

从辣椒中克隆到一个与拟南芥系统获得抗性正调节基因NPR1同源的CaNPR1基因的全长cDNA。辣椒CaNPR1基因与拟南芥NPR1基因在mRNA水平上同源性为62.9%,两者的编码产物一致性为49.7%,相似性为65.7%。为鉴定该基因的功能,构建了一个以CaNPR1基因为靶基因的RNA干涉辣椒表达载体pCaNPR1-RNAi,并用根癌农杆菌介导法转化辣椒桂研五号,共获得了6株卡那霉素抗性再生苗,经Southern杂交证实,这些再生苗均为转基因植株,为进一步鉴定该基因的功能奠定了基础。

关键词: CaNPR1基因克隆 RNA干涉辣椒遗传转化

DOI：

分类号:Q943

基金项目:

Construction of pepper CaNPR1-RNAi expression vector and its transformation to pepper

LUO Huan^1,2, DUAN Cheng-Jie^1,2, CHEN Bao-Shan^1,2 TANG Ji-Liang^1,2, FENG Jia-Xun^1,2*

1.Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning 530005, China;2.College of Life Science and Technology, Guangxi University, Nanning 530005, China 1.Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning 530005, China; 2.College of Life Science and Technology, Guangxi University, Nanning 530005, China

Abstract:

Full-length cDNA of CaNPR1,a homologous gene of the positive regulatory gene NPR1 in systemic acquired resistance of Arabidopsis thaliana,was cloned from pepper(Capsicum annuum). CaNPR1 and NPR1 share 62.9% homology at mRNA level and their encoded products have 49.7% identity and 65.7% similarity. In order to identify the function of CaNPR1 in SAR of pepper, an expression vector pCaNPR1-RNAi for silencing CaNPR1 in pepper was constructed. Pepper cultivar Guiyan5 was transformed with pCaNPR1-RNAi by Agrobacterium tumefaciens-mediated transformation. Six kanamycin-resistant transgenic seedlings were obtained. Southern analysis confirmed that all the six seedlings carried the introduced transgene.

Key words: CaNPR1 gene cloning RNA interference pepper transformation