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| 引用本文: | 禤维言, 郑学勤.一种适于提取荔枝花与幼果组织总RNA的方法[J].广西植物,2009,(1):59-61.[点击复制] |
| XUAN Wei-Yan, ZHENG Xue-Qin.A practical method for extracting total RNAfrom flower and fruit of Litchi[J].Guihaia,2009,(1):59-61.[点击复制] |
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| 一种适于提取荔枝花与幼果组织总RNA的方法 |
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禤维言1,2, 郑学勤1*
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1.中国热带农业科学院 热带作物生物技术研究所,海口 571107;2.广西大学 农学院, 南宁 530005
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| 摘要: |
| 介绍了一种从荔枝花与幼果组织中提取高质量和较高产量的总RNA的方法,该方法提取的总RNA可以满足构建cDNA文库、开展RT-PCR、Northern杂交分析、基因表达差示分析等方面研究的要求。 |
| 关键词: RNA提取 CTAB 荔枝 |
| DOI： |
| 分类号:Q943 |
| 基金项目: |
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| A practical method for extracting total RNAfrom flower and fruit of Litchi |
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XUAN Wei-Yan1,2, ZHENG Xue-Qin1*
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1.Institute of Tropical Crop Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou
571101, China;2.College of Agricultural, Guangxi University, Nanning 530005, China
1.Institute of Tropical Crop Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou
571101, China; 2.College of Agricultural, Guangxi University, Nanning 530005, China
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| Abstract: |
| A practical method for extracting high-quality and productive total RNA from flower and fruit of seedless litchi was introduced. These RNA samples thus prepared meet the needs of many molecular biological researches such as constructing cDNA library,RT-PCR,Northern blot and studies on difference analysis of gene expression. |
| Key words: RNA isolation CTAB litchi |
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