| 摘要: |
| 应用MS为基本培养基,通过各种培养条件和不同激素配比,探讨蔓花生组织培养及其植株再生条件的优化。结果显示:幼叶为最佳的外植体,在幼叶愈伤诱导过程中,不超过12 h光照,光照强度在21.6 μmol·m-2·s-1均可; 诱导愈伤组织的适宜培养基为MS+0.5 mg/L6-BA+0.2 mg/L2,4-D或MS+0.5 mg/L6-BA+2 mg/LNAA; 最适的分化培养基为MS+1 mg/LTDZ+2 mg/L6-BA+0.5 mg/LNAA; 最适的生根培养基1/2MS+1 mg/LNAA+1 mg/LPP333。 |
| 关键词: 蔓花生 组织培养 愈伤组织 |
| DOI： |
| 分类号: |
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| Condition optimization for tissue culture of Arachis duranensis |
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ZHANG Zhen-Xia, ZHENG Yu-Zhong
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Department of Biology, Hanshan Teachers College, Chaozhou 521041, China
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| Abstract: |
| Condition optimization for tissue culture and regeneration of Arachis duranensis was studied. The results showed that the leaflet was the best experimental explant; the optimized medium for callus was MS+0.5 mg/L6-BA+0.2 mg/L2,4-D or MS+0.5 mg/L6-BA+0.2 mg/LNAA with illumination of 12h/d; the optimized medium for differentiation was MS+1 mg/LTDZ+2 mg/L6-BA+0.5 mg/LNAA; the optimized medium for root induction was 1/2MS+1 mg/LNAA+1 mg/LPP333. |
| Key words: Arachis duranensis tissue culture callus |
