一个水稻OsWNK基因的分离及表达分析

王小兰<sup>1</sup>; 刘顺枝<sup>1</sup>; 何光存<sup>2</sup>; 田长恩<sup>1*</sup>

引用本文:	王小兰, 刘顺枝, 何光存, 田长恩.一个水稻OsWNK基因的分离及表达分析[J].广西植物,2009,(3):404-407.[点击复制]
	WANG Xiao-Lan, LIU Shun-Zhi, HE Guang-Cun, TIAN Chang-En.Isolation and expression analysis of an OsWNK gene in rice[J].Guihaia,2009,(3):404-407.[点击复制]

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*一个水稻OsWNK基因的分离及表达分析*
王小兰¹, 刘顺枝¹, 何光存², 田长恩^1*
1.广州大学生命科学学院植物抗逆基因功能研究广州市重点实验室, 广州 510006;2.武汉大学生命科学学院植物发育生物学教育部重点实验室, 武汉 430072

摘要:

在褐飞虱取食后的水稻cDNA差减文库中筛选到与拟南芥AtWNK1激酶基因高度同源的EST(GenBank登录号:BU572310),以该EST为探针,从褐飞虱取食后的水稻cDNA文库中分离到OsWNK基因的全长cDNA,该基因编码一个含677个氨基酸残基的蛋白激酶,与以前克隆出的一种拟南芥蛋白激酶基因(GenBank登录号:DQ837532)只有3个氨基酸残基的差异。Northern杂交结果显示,在褐飞虱取食后,该基因的表达上升。表明该激酶基因参与褐飞虱取食的应答反应,可能与水稻抗褐飞虱有关.

关键词: 褐飞虱 OsWNK cDNA筛选水稻

DOI：

分类号:Q966,Q786

基金项目:

Isolation and expression analysis of an OsWNK gene in rice

WANG Xiao-Lan¹, LIU Shun-Zhi¹, HE Guang-Cun², TIAN Chang-En^1*

1.Guangzhou Key Laboratory for Functional Studies on Plant Stress-Resistant Genes, School of Life Sciences, Guangzhou University,Guangzhou, 510006, China;2.Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China

Abstract:

BPHiw008,an EST(expressed sequence tag)which is highly homologous with Arabidopsis WNK1(with no lysine kinase),has been screeded from the rice cDNA SSH library. Using this EST as probe to screen the cDNA library induced by brown planthopper sucking,a full length of cDNA with 2.4 Kb in size was isolated and named as OsWNK. OsWNK is deduced to encode a 677-amino-acid protein OsWNK through BLAST. Only 3 amino-acid residues of OsWNK are different from those of AtWNK1. Result of RNA gel blot analysis showed that OsWNK was up-regulated in brown planthopper-sucked rice,indicating that OsWNK may play a role in resistance to brown planthopper of rice.

Key words: brown planthopper OsWNK cDNA screening rice