| 摘要: |
| 重组水母发光蛋白作为检测植物细胞钙信号的手段是近十几年发展起来的新方法,该文介绍了重组水母发光蛋白作为Ca2+检测探针的发展过程、测钙原理、Ca2+浓度检测方法、Ca2+浓度换算方法、优点与不足、及在植物细胞钙离子信号检测中的研究进展。并利用国外实验室提供的方法在国内首次得出冷激条件下植物细胞内细胞质中(〖Ca2+〗cyt)和液泡膜附近(〖Ca2+〗md)钙离子浓度动力学变化曲线。 |
| 关键词: 重组水母发光蛋白 钙浓度换算 〖Ca2+〗cyt 钙信号 |
| DOI： |
| 分类号:Q945, Q943 |
| 基金项目: |
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| Recombinant aequorin luminous protein and its application in measuring calcium signal of plant cell |
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HAO Xiao-Hua1, ZHANG Guo-Zeng2
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1.Department of Life Sciences, Hunan Art and Science College, Changde 415000, China;2.Department of Life Sciences, Henan University, Kaifeng 475000, China
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| Abstract: |
| Recently,the application of recombinant aequorin luminous protein has appeared in measuring calcium signal of plant cell as a new method. This paper introduced mainly the development of the recombinant aequorin luminous protein as a probe,the principle and operates method of measuring calcium concentration,and how to conversion the calcium concentration. It summarized the advantages and deficiencies of the protein,and collected mainly the progress in development studies in measuring calcium signal of plant cell. Meanwhile,using the method provided by other laboratory,the authors got the cold shock kinetics of 〖Ca2+〗cyt and 〖Ca2+〗md for the first time. |
| Key words: recombinant aequorin luminous protein conversion to the calcium concentration 〖Ca2+〗cyt calcium signal |
