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| 引用本文: | 蔡彩虹, 谭彩银, 陈惠琴, 王 昊, 梅文莉, 宋希强, 戴好富.华石斛化学成分研究(Ⅱ)[J].广西植物,2020,40(9):1368-1374.[点击复制] |
| CAI Caihong, TAN Caiyin, CHEN Huiqin, WANG Hao,
MEI Wenli, SONG Xiqiang, DAI Haofu.Chemical constituents from Dendrobium sinense(Ⅱ)[J].Guihaia,2020,40(9):1368-1374.[点击复制] |
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| 华石斛化学成分研究(Ⅱ) |
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蔡彩虹1,3, 谭彩银1,2, 陈惠琴1,3, 王 昊1,3, 梅文莉1,3, 宋希强2, 戴好富1,3*
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1. 海南省黎药资源天然产物研究与利用重点实验室, 中国热带农业科学院热带生物技术研究所, 海口 571101;2. 海南省
热带特色花木资源生物学重点实验室, 海南大学 林学院, 海口 570228;3. 海口市石斛工程技术研究中心, 海口 571101
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| 摘要: |
| 海南特有植物华石斛(Dendrobium sinense)的化学成分和药理活性研究报道较少。为了深入研究华石斛的化学成分,该研究采用MCI小孔树脂柱色谱、硅胶柱色谱和Sephadex LH-20柱色谱等多种现代分离纯化技术,从其全草乙醇提取物的乙酸乙酯部位中分离纯化了10个化合物。结果表明:对分离纯化得到的10个化合物鉴定为罗汉松脂素(1)、4,5-二羟基-2,3-二甲氧基-9,10-二氢菲(2)、华石斛素C(3)、对甲氧基苯乙醇(4)、顺式对羟基肉桂酸乙酯(5)、对羟基苯丙酸乙酯(6)、丁香醛(7)、3-羟基苯甲醛(8)、3,9-dihydroxy-megastigma-5-ene(9)和(9Z,12Z)-9,12-二烯十八碳酸甲酯(10)。其中,首次从华石斛中分离得到的有化合物1、4-6、8-10。通过体外抑制乙酰胆碱酯酶活性实验发现,化合物2、4和化合物 9能够使乙酰胆碱酯酶活力下降。 |
| 关键词: 华石斛, 化学成分, 乙酰胆碱脂酶抑制活性 |
| DOI：10.11931/guihaia.gxzw201907003 |
| 分类号:Q946 |
| 文章编号:1000-3142(2020)09-1368-07 |
| 基金项目:海南省自然科学基金青年基金(218QN284,2018CXTD331); 海南省重大科技计划项目(ZDKJ201805 ); 现代农业产业技术体系建设专项资金项目(CARS-21)[Supported by Hainan Provincial Natural Science Foundation of China(218QN284, 2018CXTD331); Major Technology Program of Hainan Province(ZDKJ201805); China Agriculture Research System(CARS-21)]。 |
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| Chemical constituents from Dendrobium sinense(Ⅱ) |
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CAI Caihong1,3, TAN Caiyin1,2, CHEN Huiqin1,3, WANG Hao1,3,
MEI Wenli1,3, SONG Xiqiang2, DAI Haofu1,3*
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1. Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and
Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;2. Key Laboratory of Germplasm Resources
of Tropical Special Ornamental Plant of Hainan Province, College of Forestry, Hainan University, Haikou 570228,
China;3. Haikou Engineering Research Center of Dendrobium, Haikou 571101, China
1. Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Institute of Tropical Bioscience and
Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 2. Key Laboratory of Germplasm Resources
of Tropical Special Ornamental Plant of Hainan Province, College of Forestry, Hainan University, Haikou 570228,
China; 3. Haikou Engineering Research Center of Dendrobium, Haikou 571101, China
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| Abstract: |
| As the Hainan endemic plant, Dendrobium sinense was reported rarely about their chemical constituents and pharmacological activities. In order to comprehensively study the chemical constituents of this species, various column chromatography including MCI, silica gel and Sephadex LH-20 were conducted, through which ten compounds were isolated from the ethyl acetate fraction of ethanol extraction of the whole herb. On the basis of the physical data and spectral analysis, the structures were identified as matairesinol(1), 4,5-dihydroxy-2,3-dimethoxy-9,10-dihydrophenanthrene(2), dendrosinen C(3), 4-methoxy-phenylethanol(4), eis-p-hydroxyl ethyl cinnamate(5), p-hydroxyphenylpropionic ethyl ester(6), syringaldehyde(7), 3-hydroxybenzaldehyde(8), 3,9-dihydroxy-megastigma-5-ene(9)and(9Z,12Z)-methyl octadeca-9,12-dienoate(10). Compounds 1, 4-6, 8-10 were obtained from this plant for the first time. The bioactivity results indicated that compounds 2, 4 and 9 showed a certain acetylcholinesterase inhibitory activity. |
| Key words: Dendrobium sinense, chemical constituents, acetylcholinesterase(AChE)inhibitory activity |
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