艾纳香野生种群克隆多样性及克隆结构研究

庞玉新<sup>1; 2</sup>; 王文全<sup>1*</sup>; 张影波<sup>2; 3</sup>; 莫廷辉<sup>3</sup>; 袁媛<sup>4</sup>

引用本文:	庞玉新,王文全,张影波,莫廷辉,袁媛.艾纳香野生种群克隆多样性及克隆结构研究[J].广西植物,2010,(2):209-214.[点击复制]
	PANGYu-Xin,WANGWen-Quan,ZHANGYing-Bo,MOTing-Hui,YUANYuan.Clonal diversity and structure in natrual populations of Blumea balsamifera[J].Guihaia,2010,(2):209-214.[点击复制]

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艾纳香野生种群克隆多样性及克隆结构研究
庞玉新^1,2,王文全^1*,张影波^2,3,莫廷辉³,袁媛⁴
1.北京中医药大学中药学院, 北京 100102;2.中国热带农业科学院热带作物品种资源研究所, 海南儋州, 571737;3.3.海南大学儋州校区农学院, 海南儋州 571737;4.海南大学儋州校区环植学院, 海南儋州 571737

摘要:

艾纳香是具有克隆生长习性的多年生宿根性草本植物,其广布于中国南部,为了更有效地保护和合理利用艾纳香资源,本文利用RAPD分子标记技术,对4个野生艾纳香种群进行了克隆结构和克隆多样性(单克隆种群或多克隆种群)进行了初步研究。结果表明：（1)10对10 bp随机引物共检测到70条谱带,其中多态带为60条,占85.71%,检测到64个基因型,且全部为局限基因型;(2)与Ellstrand & Roose(1987)总结的克隆多样性平均值(PD＝0.17,D＝0.62)相比艾纳香的种群克隆多样性水平稍高,Simpson指数平均为0.973,基因型比率PD平均为0.800;(3)遗传一致度和遗传距离分析表明,4个艾纳香野生种群被分成两组,一组是海南的所有种群,另外一组是云南类群。

关键词: 艾纳香克隆植物克隆多样性克隆结构遗传多样性

DOI：

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Clonal diversity and structure in natrual populations of Blumea balsamifera

PANGYu-Xin^1,2,WANGWen-Quan^1*,ZHANGYing-Bo^2,3,MOTing-Hui³,YUANYuan⁴

1.College of Chinese Medical Sciences, Beijing University of Traditional Chinese Medicine, Beijing100102, China;2. 2.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China;3.College of Agriculture, Hainan University, Danzhou 571737, China;4.College of Environment and Plant Protection, Hainan University, Danzhou 571737, China

Abstract:

Ramet of Blumea balsamifera belongs to perennial herbaceous plant,which widely distributes in south of China. The objectives of the present study were to assess the clonal structure and diversity(monoclonal or multiclonal population)on wild populations of B.balsamifera for the purpose of efficient conservation and reasonable utilization of its resources. Eighty individuals of B.balsamifera were collected from four natural populations in different and typical habitats in its major distribution areas in China,and they were studied byRAPD analysis. Seventy loci were detected by using 10 random primers(10bp),and 60 of them were found polymorphic(85.71%). Sixty four RAPD genotypes were differentiated among the 80 plants sampled. The mean Simpson’s index was 0.973,and mean PD was 0800,slightly higher than the mean of Ellstrand & Roose(PD=0.17,D=0.62). With UPGMA cluster analysis,four natural B.balsamifera populations can be divided into two clusters,one is all the Hainan populations,and the other is the Yunnan populaitions.

Key words: Blumea balsamifera clonal plants clonal diversity clonal structure genetic diversity