| 引用本文: | 陈文娟,李典鹏,陈月圆,黄永林,文永新.半制备高效液相色谱制备5种苯乙醇苷类单体[J].广西植物,2010,(2):269-273.[点击复制] |
| CHENWen-Juan,LIDian-Peng,CHENYue-Yuan,HUANGYong-Lin,WENYong-Xin.Preparation of five phenylethanoid glycoside components by semi preparative HPLC[J].Guihaia,2010,(2):269-273.[点击复制] |
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| 摘要: |
| 利用MCI柱层析和半制备型反相高效液相色谱法从牛耳朵中分离制备了5种苯乙醇苷类单体。在Eclipse XDB C18(9.4 mm×250 mm,5 μm)半制备柱上,以甲醇 水为流动相,流速为4 mL/min,采用梯度洗脱方式,制备得到5个组分。经波谱(EI-MS,1H-NMR、13C-NMR)分析,分别鉴定为plantainoside(1)、chiritoside C(2)、plantaninoside B(3)、plantamajoside(4)和desrhamnosylverbascoside(5)。高效液相色谱分析表明所制备5种化合物纯度均高于98%。该方法分离条件简单,高效,一次可得到5个单体,目标产物纯度较高。 |
| 关键词: 半制备高效液相色谱 牛耳朵 苯乙醇苷类 |
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| Preparation of five phenylethanoid glycoside components by semi preparative HPLC |
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CHENWen-Juan1,2,LIDian-Peng1,CHENYue-Yuan1,HUANGYong-Lin1,WENYong-Xin1*
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1.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China;2.College of Life Sciences, Guangxi Normal University, Guilin 541004, China
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| Abstract: |
| A method was established for the isolation and preparation of five phenylethanoid glycoside components from Chirita eburnea using MCI column chromatography and semi preparative HPLC. A semi preparative Eclipse XDB C18 (9.4 mm×250 mm,5 μm) was used in the preparation process. By gradient elution with methanol water as the mobile phase at a flow rate of 4 mL/min,five phenylethanoid glycoside components were obtained from Chirita eburnea,and on the basis of spectral data (EI-MS,1H-NMR,13C-NMR),these compounds were identified as plantainoside(1),chiritoside C(2),plantaninoside B(3),plantamajoside(4) and desrhamnosylverbascoside(5),and the purities of these five phenylethanoid glycoside components analyzed by HPLC were all beyond of 98%. The method is efficient,simple with high purification and got 5 components for one time. |
| Key words: semi-preparative HPLC Chirita eburnea phenylethanoid glycosides |
