云南真藓和细叶真藓的核糖体DNA ITS序列分析

汪琛颖<sup>1; 2</sup>; 李利博<sup>1</sup>; 赵建成<sup>1*</sup>

引用本文:	汪琛颖, 李利博, 赵建成.云南真藓和细叶真藓的核糖体DNA ITS序列分析[J].广西植物,2011,(1):25-30.[点击复制]
	WANG Chen-Ying, LI Li-Bo, ZHAO Jian-Cheng.ITS sequences analysis of nuclear ribosomal DNA of Bryum yuennanense and B.capillare[J].Guihaia,2011,(1):25-30.[点击复制]

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云南真藓和细叶真藓的核糖体DNA ITS序列分析
汪琛颖^1,2, 李利博¹, 赵建成^1*
1.河北师范大学生命科学学院, 石家庄 050016;2.郑州师范学院生命科学系, 郑州 450044

摘要:

从形态特征和地理分布特征比较分析云南真藓和细叶真藓的区别,通过对核糖体DNA的内转录间隔区序列分析研究,对二者进行分子水平鉴定。对包括真藓属及相关属的6种植物的ITS序列进行扩增和测序,使用DNAMAN和MEGA3.0软件对获得的ITS序列数据进行分析。结果表明,云南真藓和细叶真藓的ITS-1和ITS-2区在序列长度,G+C含量上均存在差异。UPGMA法聚类分析表明,这种差异发生在种级水平。研究结果表明,云南真藓和细叶真藓应视为独立的两个种。

关键词: 细叶真藓云南真藓 ITS序列序列分析

DOI：10.3969/j.issn.1000-3142.2011.01.007

分类号:Q944.1

基金项目:

ITS sequences analysis of nuclear ribosomal DNA of Bryum yuennanense and B.capillare

WANG Chen-Ying^1,2, LI Li-Bo¹, ZHAO Jian-Cheng^1*

1.College of Life Science, Hebei Normal University, Shijiazhuang 050016, China;2.Department of Life Science, Zhengzhou Normal University, Zhengzhou 450044, China

Abstract:

Morphological characteristics and ecological distributions of Bryum yuennanense and B.capillare were analyzed in this study. Molecular identifications were done by the analysis of Bryum yuennanense and B.capillare ITS region of the six species in the related genus were amplified and sequenced. The sequences data were analyzed by DNAMAN and MEGA3.0. The results showed that the length of sequences and content of G+C of ITS-1 and ITS-2 were different between B.yuennanense and B.capillare. Phylogenetic tree based on ITS sequences data was conducted by UPGMA method,which showed some variations at the level of species. B.yuennanense and B.capillare should be assigned as two different taxa.

Key words: Bryum capillare Bryum yuennanense ITS sequence sequence analysis