盐肤木基因组DNA提取方法改进及AFLP体系的建立

庞雅文<sup>1</sup>; 段立柱<sup>1</sup>; 宋德应<sup>2</sup>; 任竹梅<sup>1*</sup>

引用本文:	庞雅文, 段立柱, 宋德应, 任竹梅.盐肤木基因组DNA提取方法改进及AFLP体系的建立[J].广西植物,2011,(1):36-38.[点击复制]
	PANG Ya-Wen, DUAN Li-Zhu, SONG De-Ying, REN Zhu-Mei.A modified method of genomic DNA extraction and establishment of AFLP systems in Rhus chinensis[J].Guihaia,2011,(1):36-38.[点击复制]

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盐肤木基因组DNA提取方法改进及AFLP体系的建立
庞雅文¹, 段立柱¹, 宋德应², 任竹梅^1*
1.山西大学生命科学学院, 太原 030006;2.湖北五峰土家族自治县林业局, 湖北五峰 443400

摘要:

经过反复试验,摸索出一种提取高质量植物基因组DNA的方法:改良的4×CTAB法。以盐肤木叶片为实验材料,提取到高质量的基因组DNA,建立了酶切、连接、预扩增、选择性扩增的AFLP反应体系。通过两种引物组合“E+3/M+3”和“E+2/M+3”策略筛选出8对条带分辨率高、多态性好的引物组合,优化了盐肤木的AFLP银染反应体系,得到了清晰的AFLP指纹图谱,为盐肤木种群遗传多样性研究奠定了基础。

关键词: 盐肤木 DNA提取 4×CTAB法 AFLP技术

DOI：10.3969/j.issn.1000-3142.2011.01.008

分类号:Q943.2

基金项目:

A modified method of genomic DNA extraction and establishment of AFLP systems in Rhus chinensis

PANG Ya-Wen¹, DUAN Li-Zhu¹, SONG De-Ying², REN Zhu-Mei^1*

1.School of Life Science, Shanxi University, Taiyuan 030006, China;2.Bureau of Forestry of Wufeng County, Wufeng 443400, China

Abstract:

A modified method(4×CTAB)was used to extract the effective genomic DNA from the leaves of Rhus chinensis,and the systems of DNA restriction reaction,ligase reaction,pre-amplification and selective amplification reaction were established in this study. Using the two kinds of primer-combined methods(E+3/M+3 and E+2/M+3),8 pairs of primers were finally selected to amplify the genomic DNA of R.chinensis,and clear bands with high genetic polymorphism and difference were obtained. The present results provided a strong foundation for the further examination of population genetic diversity and structure of R.chinensis using AFLP method.

Key words: Rhus chinensis DNA extraction 4×CTAB method AFLP technology