基于AFLP分子标记技术的黑花韭与多星韭遗传多样性与亲缘关系分析

王 锦<sup>1</sup>; 何承中<sup>1</sup>; 党承林<sup>2</sup>; 黄瑞复<sup>2</sup>

引用本文:	王锦, 何承中, 党承林, 黄瑞复.基于AFLP分子标记技术的黑花韭与多星韭遗传多样性与亲缘关系分析[J].广西植物,2011,(3):295-299.[点击复制]
	WANG Jin, HE Cheng-Zhong, DANG Cheng-Lin, HUANG Rui-Fu.Genetic diversity and relationship of Allium tchongchanense and A.wallichii based on AFLP analysis[J].Guihaia,2011,(3):295-299.[点击复制]

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基于AFLP分子标记技术的黑花韭与多星韭遗传多样性与亲缘关系分析
王锦¹, 何承中¹, 党承林², 黄瑞复²
1.西南林业大学, 昆明 650224;2.云南大学生命科学学院, 昆明 650031

摘要:

采用AFLP分子标记技术分析了分布在滇中、滇西、滇西北的3个黑花韭居群、3个二倍体多星韭居群和6个四倍体多星韭居群的遗传多样性与亲缘关系。结果表明,12个居群在DNA水平上已发生明显分化。居群间的基因分化系数(G_ST)为0.444。由G_ST计算的居群间基因流(N_m)为0.625,表明居群间部分基因流动受阻。12个居群间的遗传距离(D)在0.019～0.298之间,平均为0.185。UPGMA聚类结果表明居群聚类关系与地理分布相近似。四倍体多星韭与黑花韭的遗传距离较与二倍体多星韭的遗传距离要小。二倍体多星韭与黑花韭的遗传距离最大,它们之间的亲缘关系最远。

关键词: 黑花韭多星韭遗传多样性亲缘关系

DOI：10.3969/j.issn.1000-3142.2011.05.004

分类号:Q152; Q157

基金项目:

Genetic diversity and relationship of Allium tchongchanense and A.wallichii based on AFLP analysis

WANG Jin¹, HE Cheng-Zhong¹, DANG Cheng-Lin², HUANG Rui-Fu²

1.Southwest Forestry University, Kunming 650224, China;2.College of Life Science, Yunnan University, Kunming 650031, China 1.Southwest Forestry University, Kunming 650224, China; 2.College of Life Science, Yunnan University, Kunming 650031, China

Abstract:

Genetic diversity and relationship of 12 populations of Allium tchongchanense and A.wallichii which distributed in the central region,western region and northwestern region of Yunnan Province were analyzed by AFLP. The results showed that there was obvious genetic differentiation among populations. Genetic differentiation coefficient G_ST=0.444. N_m=0.625 calculated by G_ST showed that gene flow was interrupted among populations. The genetic distance(D)of 12 populations was 0.019-0.298,average 0.185. UPGMA cluster results showed that genetic distance relationship was in accordance with geographic distribution. The genetic distance between A.wallichii tetraploid and A.tchongchanenseow was smaller than that between A.wallichii tetraploid and A.wallichii diploid. The genetic distance was the largest between A.wallichii diploid and A.tchongchanense.

Key words: Allium tchongchanense A.wallichii genetic diversity relationship