薇甘菊可溶性蛋白和抗氧化酶活性对田野菟丝子不同寄生密度的响应

刘梦佼1; 2*; $21; 2*; $21**; $23; $21; $21

引用本文:	刘梦佼1，2，洪岚1，2，沈浩1**，韦霄3，叶万辉1，曹洪麟1.薇甘菊可溶性蛋白和抗氧化酶活性对田野菟丝子不同寄生密度的响应[J].广西植物,2011,(4):520-525.[点击复制]
	LIU Meng Jiao1，2， HONG Lan1，2， SHEN Hao1**， WEI Xiao3， YE Wan Hui1， CAO Hong Lin1.Responses of Mikania micrantha to parasitization of Cuscuta campestris in total soluble protein content and activities of antioxidant enzymes[J].Guihaia,2011,(4):520-525.[点击复制]

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薇甘菊可溶性蛋白和抗氧化酶活性对田野菟丝子不同寄生密度的响应
刘梦佼1，2，洪岚1，2，沈浩1**，韦霄3，叶万辉1，曹洪麟1
1.中国科学院华南植物园，广州 510650;2.中国科学院研究生院，北京 100049;3.3.广西壮族自治区中国科学院　广西植物研究所，广西桂林 541006

摘要:

为探求利用寄生植物田野菟丝子对入侵杂草薇甘菊进行生物控制的有效措施，研究了薇甘菊对0、1、2、4和8棵田野菟丝子幼苗寄生在可溶性蛋白和一些抗氧化酶活性方面的响应。寄生后30 d，1棵田野菟丝子/株薇甘菊（以下简称棵/株）以上的寄生密度导致薇甘菊可溶性蛋白含量显著降低。和对照相比，在寄生密度为1棵/株时，超氧物歧化酶（SOD）和过氧化物酶（POD）活性显著增强；但随着寄生密度的加大而下降，而且在寄生密度为4棵/株时，SOD和POD分别等于和小于对照，在8棵/株时均显著小于对照。在各寄生密度下，寄主的过氧化氢酶（CAT）活性均小于对照，而SOD/CAT，SOD/POD和SOD/（CAT+POD）比率均大于对照。这些结果表明，田野菟丝子的寄生对薇甘菊可溶性蛋白和抗氧化酶活性的影响依赖于寄生密度，在野外利用田野菟丝子控制薇甘菊的最理想寄生密度是4棵/株，从而可为野外利用田野菟丝子控制薇甘菊的技术体系提供参考。

关键词: 生物防治入侵杂草寄生植物抗氧化酶

DOI：

分类号:Q945.79

基金项目:

Responses of Mikania micrantha to parasitization of Cuscuta campestris in total soluble protein content and activities of antioxidant enzymes

LIU MengJiao1，2*， HONG Lan1，2*， SHEN Hao1**， WEI Xiao3， YE WanHui1， CAO HongLin1

1.South China Botanical Garden， Chinese Academy of Sciences， Guangzhou 510650， China;2.Graduate School of the Chinese Academy of Sciences， Beijing 100049， China;3.Guangxi Institute of Botany， Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences， Guilin 541006， China

Abstract:

To develop efficient biocontrol techniques for the invasive weed Mikania micrantha，the use of the obligate parasitic plant，Cuscuta campestris Yuncker as a biological control was investigated. In this experiment，whether the impacts of the parasite on host soluble protein content and activities of some antioxidant enzymes were affected by the density of the parasite was tested. The responses of M.micrantha to parasitic densities of 0，1，2，4 and 8 individual seedlings of C.campestris per host plant were examined.On the 30th day of parasitization，infection with more than 1 C.campestris seedling significantly lowered soluble protein content. Compared with control，activities of superoxide dismutase (SOD) and peroxidase (POD) significantly increased at parasitic density of 1 parasite per host plant；but as parasitic density increased，both SOD and POD activities decreased.Moreover，SOD activity was not changed but POD activity was significantly decreased at parasitic density of 4 parasites per host，and both SOD and POD activities were significantly decreased at parasitic density of 8 parasites per host，compared with control. The infected plants had significantly lower catalase (CAT) activity but higher SOD/CAT，SOD/POD and SOD/ (CAT+POD) ratios than the control at all parasitic densities. The results indicated that the effects of C.campestris infection on M.micrantha were density dependent，which provided a basis for refining this strategy for biological control of M.micrantha. The optimal cost effective number of parasites to control M.micrantha was 4 per host plant in the field.

Key words: biological control invasive weed parasitic plant antioxidant enzymes