巫山淫羊藿愈伤组织细胞的悬浮培养条件优化的初步研究

韩素菊1; $22*

引用本文:	韩素菊1，黎云祥2*.巫山淫羊藿愈伤组织细胞的悬浮培养条件优化的初步研究[J].广西植物,2011,(6):827-831.[点击复制]
	HAN Su Ju1， LI Yun Xiang2*.Primary study on suspension cell culture of Epimediun wushanense[J].Guihaia,2011,(6):827-831.[点击复制]

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巫山淫羊藿愈伤组织细胞的悬浮培养条件优化的初步研究
韩素菊1，黎云祥2*
1.绵阳师范学院，四川绵阳 621000;2.西华师范大学，西南野生动植物资源保护教育部重点实验室，四川南充 637002

摘要:

通过研究接种量、激素配比、糖浓度、培养基种类对巫山淫羊藿悬浮培养细胞生长及其愈伤组织黄酮类含量的影响，建立了巫山淫羊藿细胞悬浮培养的技术体〖JP2〗系。结果表明：巫山淫羊藿愈伤组织细胞悬浮培养在B5基本培养基中并附加1.0 mg•L 12，4 D和0.2 mg•L 1BA，蔗糖浓度40 g•L 1，接〖JP〗种量每30 mL为鲜重2 g时，黄酮类化合物含量最高。说明用松脆的巫山淫羊藿胚性愈伤组织可容易建立理想的悬浮细胞培养体系。

关键词: 巫山淫羊藿细胞悬浮培养黄酮类化合物

DOI：

分类号:Q945

基金项目:

Primary study on suspension cell culture of Epimediun wushanense

HAN SuJu1， LI YunXiang2*

1.Mianyang Normal University， Mianyang 621000， China;2.ChinaWest Normal University， Key Laboratory of Southwest China Wildlife Resources Conservation， Nanchong 637002， China

Abstract:

The technological system of cell suspension culture by the flavonoids content of E.wushanense was established through studing the effect of inoculation concentration，hormone combination，sugar concentration and various medias. The result showed that the suspension culture optimum medium of E. wushanense was B5 medium supplemented with 1.0 mg•L 12，4 D and 0.2 mg•L 16 BA，the sugar concentration was 4.0% and the optimum inoculation concentration was 2 g per 30 mL. The highest content of the total flavonoids appeared on the medium.

Key words: Epimediun wushanense suspension cell culture flavonoids