| 摘要: |
| 基于RACE技术克隆得到尾巨桉3 羟基 3 甲基戊二酰CoA还原酶（EuHMGR）基因全长为1 955 bp，包含1 560 bp的开放阅读框（ORF），编码519个氨基酸。生物信息学分析表明EuHMGR包含两个HMG CoA结合基序和两个NADP(H)结合基序；同源建模得到EuHMGR三维构象呈“V”型，包含N 结构域、S 结构域和L 结构域。将EuHMGR组DNA与cDNA序列进行比对表明EuHMGR存在一个319 bp的内含子。通过实时定量PCR进行组织特异性表达分析表明EuHMGR在茎中的表达量最高，叶次之，在根中基本无表达。该研究为后续尾巨桉EuHMGR的功能验证以及遗传转化奠定基础。 |
| 关键词: 尾巨桉 HMGR 生物信息学 内含子 组织特异性表达 |
| DOI： |
| 分类号:Q943.2；Q946.85 |
| 基金项目: |
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| Molecular Cloning and expression analysis of 3 hydroxy 3 methylglutaryl CoA Reductase Gene from Eucalyptus urophylla×E.grandis |
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JIANG Chun1， ZHANG HuaLing2， PENG Jiang2*
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1.Key Laboratory of Bioresources and Ecoenvironment of Ministry of Education， School of Life Sciences, Sichuan University， Chengdu 610064， China;2.Key Laboratory of Ecoenvironments in Three Gorges Reservoir Region， Ministry of Education， School of Life Sciences， Southwest University， Chongqing 400715， China
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| Abstract: |
| The gene encoding 3 hydroxy 3 methylglutaryl CoA reductase (HMGR)was cloned by Rapid Amplification of cDNA Ends (RACE)method，the full length EuHMGR cDNA was 1955 bp，containing a 1 560 bp open reading frame，encoding a peptide of 519 amino acids. Bioinformatics analysis indicated EuHMGR contained two HMG CoA binding motifs and two NADP(H)binding motifs；and it had a 3 D structure with“V”homology based on modeling，containing N domain，S domain and L domain. There was a 319 bp intron in EuHMGR gene genomic DNA sequence compared with the cDNA sequence. Tissue expression profile analysis by Real Time quantitative PCR indicated EuHMGR expression was the highest in stem，followed by leaf 〖JP3〗and no expression in root. The paper would supply some information to the function analysis and genetic transformation of EuHMGR from Eucalyptus urophylla×E.grandis. |
| Key words: Eucalyptus urophyla×E.grandis HMGR bioinformatics intron tissue specific expression |
