| 摘要: |
| 简单重复序列也称为微卫星分子标记，不仅在同属近缘种间具有良好的通用性，甚至在近缘属间也具有一定的通用性。本研究利用壳斗科基因组信息数据库中公布的中国板栗124对多态的EST SSR引物在栲树中进行跨属(栗属到栲属)通用性研究，结果显示中国板栗EST SSR引物在栲树中通用性和多态性分别为42.7%和56.6%；使用19对多态的EST SSR引物对4个栲树自然居群的遗传多样性进行初步分析，结果显示栲树自然居群具有较高的遗传多样性（Na=6.105，Ho=0.563，He=0.621）。这些引物为栲树群体遗传学的深入研究提供了有力工具。 |
| 关键词: 中国板栗 栲树 EST SSR 通用性 |
| DOI： |
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| Transferability analysis of EST SSR markers of Castanea mollissima to Castanopsis fargesii |
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LI Chun1，2， SUN Ye1，3*
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1.South China Botanical Garden， Chinese Academy of Sciences， Guangzhou 510650， China;2.Graduate School of Chinese Academy of Sciences， Beijing 100049， China;3.Key Laboratory of Plant Resources Conservation and Sustainable Utilization， South China Botanical Garden， Chinese Academy of Sciences， Guangzhou 510650， China
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| Abstract: |
| Simple sequence repeats(SSR)，also known as microsatellite molecular markers，have been cross amplified successfully in closely related species of the same genus and even across genera within the same family. In the present study，124 primer pairs of polymorphic EST SSR originally developed from Castanea mollissima were cross amplified in Castanopsis fargesii. Results indicated that 42.7% of Castanea mollssima EST SSR primers were successfully cross amplified in Castanopsis fargesii and 56.6% were polymorphic. The genetic diversity of 4 populations of C.fargesii were investigated with polymorphic EST SSRs，preliminary results showed that C.fargesii possessed high levels of genetic diversity(Na=6.105，Ho=0.563，He=0.621). These polymorphic EST SSR primers would provide a powerful tool for further investigation on population genetics of C.fargesii. |
| Key words: Castanea mollissima Castanopsis fargesii EST SSR transferability |
