| 引用本文: | 周俊辉, 王燕平, 陈 勤, 周志钦.用AFLP分析广东省鲜食橄榄品种资源遗传多样性[J].广西植物,2014,(1):4-9.[点击复制] |
| ZHOU Jun-Hui, WANG Yan-Ping, CHEN Qin, ZHOU Zhi-Qin.Genetic diversity analysis with AFLP on fresh Chinese olive cultivars resources in Guangdong Province[J].Guihaia,2014,(1):4-9.[点击复制] |
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| 用AFLP分析广东省鲜食橄榄品种资源遗传多样性 |
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周俊辉, 王燕平, 陈 勤, 周志钦
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1. 仲恺农业工程学院 园艺园林学院, 广州 510225;2. 广东省潮洲市茶叶科学
研究中心, 广东 潮州 515726;3. 西南大学 园艺园林学院, 重庆 400716
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| 摘要: |
| 从64对引物组合中筛选出6对谱带清晰、多态性高的引物组合,对63个橄榄品种扩增,共扩增出417条多态性电泳谱带,多态性比率达100%,揭示了橄榄品种的遗传多样性。利用UPGMA对供试橄榄品种聚类,建立AFLP聚类图。结果表明:63份材料之间的遗传相似性系数为0.0606～0.7619,相似系数为0.312时,可将供试材料分为7个品种群,其中第1群包括45个品种,以‘潮阳甜榄'为代表,还可细分成3个组; 第2群包括5个品种,以‘大纳甜'为代表; 第3群包括4个品种,以‘潮阳三棱'为代表; 第4群包括‘土甜'、‘文祠三棱'和‘早花三棱'3个品种; 第5群包括‘铁条'和‘细粒'两个品种; 第6群包括‘广太甜种'、‘西土'、‘鸡心'和‘车酸1号'4个品种; 第7群只有‘凤湖橄榄'1个品种,与其他品种群亲缘关系较远,可能发生变异。 |
| 关键词: AFLP 橄榄 遗传多样性 亲缘关系 |
| DOI：10.3969/j.issn.1000-3142.2014.01.002 |
| 分类号:Q949 |
| 基金项目: |
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| Genetic diversity analysis with AFLP on fresh Chinese olive cultivars resources in Guangdong Province |
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ZHOU Jun-Hui1*, WANG Yan-Ping1,3, CHEN Qin2, ZHOU Zhi-Qin3
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1. College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering,
Guangzhou 510225, China;2. Chaozhou Institute Center of Tea, Chaozhou 515726, China;3. College of
Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China
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| Abstract: |
| Screened from 64 pairs of the AFLP primer,six pairs(E/AAC-M/CAT,E/AAC-M/CTT,E/ACA-M/CAA,E/AGG-M/CAA,E/ACC-M/CAA,E/ACT-M/CAT),which produced clear and reliable polymorphic bands,were selected for analysis genetic relationship and genetic diversity among the 63 Chinese olive cultivars. 417 amplification bands were produced with 100% polymorphic rate. It was indicated that there was rich genetic diversity in Chinese olive cultivar germplasm. The genetic relationship of the 63 Chinese olive cultivars was analyzed with software NTSYSpc-2.10a,and a UPGMA tree was established. According to this tree,the Chinese olive cultivars in this studied could be divided into 7 cultivar groups with a similar coefficient 0.312,the Group 1 delegated by ‘Chaoyang sweet olive' including 45 cultivars,could be divided into 3 types,the Group 2 delegated by ‘Danatian' including 5 cultivars,the Group 3 delegated by ‘Chaoyang sanleng' including 4 cultivars,the Group 4 including 3 cultivars,the Group 5 including 2 cultivars,the Group 6 including 4 cultivars,and the Group 7 including one cultivar only,that was ‘Fenghu olive',which had a long distance in relationship with other cultivars,maybe occurred variation in cultivation practice. |
| Key words: AFLP fresh Chinese olive genetic diversity |
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