银杏愈伤组织诱导的多因子正交试验研究

刘建军; 龚一富; 王世安; 赵群芬; 钱 丹

引用本文:	刘建军, 龚一富, 王世安, 赵群芬, 钱丹.银杏愈伤组织诱导的多因子正交试验研究[J].广西植物,2014,(1):116-119.[点击复制]
	LIU Jian-Jun, GONG Yi-Fu, WANG Shi-An, ZHAO Qun-Fen, QIAN Dan.Callus induction of Ginkgo biloba by orthogonal test[J].Guihaia,2014,(1):116-119.[点击复制]

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银杏愈伤组织诱导的多因子正交试验研究
刘建军, 龚一富, 王世安, 赵群芬, 钱丹
1. 宁波市沁香园农业开发有限公司, 浙江宁波 315800;2. 宁波大学海洋学院应用海洋生物技术教育部重点实验室, 浙江宁波 315211;3. 宁波市合一农业科技开发有限公司, 浙江宁波 315000

摘要:

采用正交试验设计法,研究了NAA、KT、2,4-D、蔗糖浓度和不同外植体类型等因素对银杏愈伤组织诱导的影响。结果表明:不同外植体类型对银杏愈伤组织诱导率影响最大,KT和NAA其次,2,4-D和蔗糖浓度最小。银杏愈伤组织诱导最佳培养基为MS+NAA 0.5 mg·L^-1+KT 0.5 mg·L^-1+蔗糖40 g·L^-1,最佳外植体为茎段,其愈伤组织诱导率可达100%。

关键词: 银杏愈伤组织诱导正交试验

DOI：10.3969/j.issn.1000-3142.2014.01.022

分类号:Q943.1

基金项目:

Callus induction of Ginkgo biloba by orthogonal test

LIU Jian-Jun¹, GONG Yi-Fu^2*, WANG Shi-An¹, ZHAO Qun-Fen², QIAN Dan³

1. Ningbo Qinxiangyuan Agriculture Development Co. Ltd., Ningbo 315800, China;2. School of Marine Science, Key Laboratory of Applied Marine Biotechnology, Minister of Education,Ningbo University, Ningbo 315211, China;3. Ningbo Heyi Agriculture Science and Technology Development Co. Ltd., Ningbo 315000, China

Abstract:

Effects of NAA,KT,2,4-D,sucrose concentration and explants type on the callus induction of Ginkgo biloba were investigated by orthogonal test in this paper. The results showed that explants type was the most important factor among the five factors for callus induction of G. biloba,followed by KT and NAA concentration,while 2,4-D and sucrose concentration were the weakest. The best medium for callus induction of G. biloba was MS+NAA 0.5 mg·L^-1+KT 0.5 mg·L^-1+sucrose 40 g·L^-1,and stem was the best explants. With the stem as explants and with the optimal medium as the culture medium,the highest rate of callus induction in G. biloba was up to 100%.

Key words: Ginkgo biloba callus induction orthogonal test