铁筷子提取物对肿瘤细胞增殖及COX-2 mRNA表达的抑制作用

曹 威; 李 娟; 程 伟; 江仁望

引用本文:	曹威, 李娟, 程伟, 江仁望.铁筷子提取物对肿瘤细胞增殖及COX-2 mRNA表达的抑制作用[J].广西植物,2014,(2):189-193.[点击复制]
	CAO Wei, LI Juan, CHENG Wei, JIANG Ren-Wang.Inhibitory effect of Helleborus thibetanus extracts on the proliferation of tumor cells and expression of COX-2 mRNA[J].Guihaia,2014,(2):189-193.[点击复制]

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铁筷子提取物对肿瘤细胞增殖及COX-2 mRNA表达的抑制作用
曹威, 李娟, 程伟, 江仁望
1. 暨南大学药学院中药及天然药物研究所, 广州 510632;2. 广西植物功能物质研究与利用重点实验室, 广西植物研究所, 广西桂林 541006;3. 湖北中医药大学药学院, 武汉430065

摘要:

研究不同铁筷子提取物对肿瘤细胞增殖及COX-2 mRNA表达的抑制作用。以铁筷子醇总提取物(TKZ1)、正丁醇萃取部位(TKZ2)、乙酸乙酯萃取部位(TKZ3)分别作用于DU145、PC3、HeLa、HT-29、HepG2等肿瘤细胞,应用噻唑蓝实验(MTT法)计算其对细胞增殖的抑制作用,应用荧光定量PCR技术检测TKZ1、TKZ2、TKZ3处理后的各肿瘤细胞中COX-2 mRNA的表达情况。结果表明:TKZ1、TKZ2、TKZ3均能显著抑制多种肿瘤细胞的增殖,与阴性对照组比较,其可以在mRNA水平上抑制COX-2的表达,且呈明显的量效关系。说明铁筷子提取物对体外肿瘤细胞的增殖具有显著的抑制作用,其抗瘤机制可能与抑制肿瘤细胞中COX-2 mRNA的表达有关。

关键词: 铁筷子抗肿瘤细胞增殖 COX-2

DOI：10.3969/j.issn.1000-3142.2014.02.012

分类号:R285

基金项目:

Inhibitory effect of Helleborus thibetanus extracts on the proliferation of tumor cells and expression of COX-2 mRNA

CAO Wei¹, LI Juan^2,3, CHENG Wei³, JIANG Ren-Wang^1,2*

1. Institute of Traditional Chinese Medicine &2.Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China;3.2. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guilin 541006, China;4.3. College of Pharmacy, Hubei University of Traditional Chinese Medicine, Wuhan 430065, China

Abstract:

To investigate the inhibitory effect of extracts from Helleborus thibetanus on cell proliferation and expression of COX-2 mRNA, the inhibitory effects of the total ethanol extract(TKZ1),n-butanol fraction(TKZ2),and ethyl acetate fraction(TKZ3)on DU145,PC3,HeLa,HT-29 and HepG2 tumor cell lines were examined by MTT assay,and the COX-2 mRNA expression levels were analyzed by quantitative PCR. The results showed that TKZ1,TKZ2 and TKZ3 could significantly inhibit the proliferation of tumor cell lines and suppress the expression of COX-2 on the mRNA level in a dose-dependent manner compared with the control. All three samples from Helleborus thibetanus (TKZ)could significantly inhibit the proliferation of tumor cells in vitro,and the mechanism might be related to the inhibition of the COX-2 mRNA expression in tumor cells.

Key words: Helleborus thibetanus antitumor cell proliferation COX-2