| 摘要: |
| OsWRKY转录因子在水稻非生物胁迫和抗病反应中具有相当重要的调节作用。为阐明其调节作用提供依据,研究了疑似功能广泛的OsWRKY转录因子表达谱,采用五个OsWRKY转录因子基因,即OsWRKY7、OsWRKY11、OsWRKY30、OsWRKY70和OsWRKY89,利用real-time PCR研究各种非生物胁迫和稻瘟菌胁迫诱导表达特征,以及各种激素对OsWRKY表达量的影响。所采用的五个基因均受到稻瘟菌胁迫的诱导,而且各种非生物胁迫也能不同程度地诱导其表达。在各个激素处理下,有些被诱导或被抑制,也有未受影响。五个OsWRKY基因均有可能参与稻瘟病胁迫响应。其中OsWRKY7和OsWRKY70可能是在JA和SA相互拮抗调控下参与,OsWRKY89可能是通过非本研究涉及的其他激素途径参与。在非生物胁迫方面,OsWRKY7可能通过ABA途径参与干旱、高盐和极端温度胁迫; OsWRKY11有可能参与高盐胁迫; OsWRKY30有可能参与高盐和高温胁迫; OsWRKY70可能参与高盐、干旱和极端温度胁迫; OsWRKY89可能参与高温胁迫,但并不是通过本研究所涉及的四种激素途径。 |
| 关键词: OsWRKY 转录因子 稻瘟病胁迫 非生物胁迫 实时定量PCR 基因表达 |
| DOI：10.3969/j.issn.1000-3142.2014.02.021 |
| 分类号:Q945.78 |
| 基金项目: |
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| Gene expression analysis of five OsWRKY transcription factors induced by abiotic and rice blast stresses |
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XU Zhan, LIN Liang-Bin*
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College of Agriculture and Biotechnology, Yunnan Agriculture University, Kunming 650201, China
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| Abstract: |
| OsWRKY transcription factors have vital function in respond to adverse environment and pathogens in rice. In order to elucidate the regulatory role of these genes,we used Realtime-PCR to investigate expression profiles of five OsWRKY transcription factors(OsWRKY7,OsWRKY11,OsWRKY30,OsWRKY70 and OsWRKY89)under abiotic stresses and pathogen challenges. The expression characteristics of hormone treatments were also investigated in order to study which hormone way they were functioning through. All five OsWRKY could be induced by pathogen inoculation and all these genes had different rate inductions under abiotic stress. In each hormone treatment,some were induced,others were suppressed,and still others unaffected. Five OsWRKY genes were all likely to be involved in the infection process of rice blast stress. OsWRKY7 and OsWRKY70 might participate in the resistance to rice blast by JA and SA in a mutually antagonistical manner; OsWRKY89 also might be involved in it,but not through the four hormone ways studied in this research. In the aspect of abiotic stress,OsWRKY7 might participate in drought,high salt and extreme temperature stresses through the ABA way; OsWRKY11 had the possibility to be involved in high salt stress; OsWRKY30 was possible to participate in high salt and hot stresses; OsWRKY70 might be involved in high salt,drought and extreme temperature stresses; OsWRKY89 might be involved in hot stress,but not through the four hormone ways studied in this research.OsWRKY transcription factors have vital function in respond to adverse environment and pathogens in rice. In order to elucidate the regulatory role of these genes,we used Realtime-PCR to investigate expression profiles of five OsWRKY transcription factors(OsWRKY7,OsWRKY11,OsWRKY30,OsWRKY70 and OsWRKY89)under abiotic stresses and pathogen challenges. The expression characteristics of hormone treatments were also investigated in order to study which hormone way they were functioning through. All five OsWRKY could be induced by pathogen inoculation and all these genes had different rate inductions under abiotic stress. In each hormone treatment,some were induced,others were suppressed,and still others unaffected. Five OsWRKY genes were all likely to be involved in the infection process of rice blast stress. OsWRKY7 and OsWRKY70 might participate in the resistance to rice blast by JA and SA in a mutually antagonistical manner; OsWRKY89 also might be involved in it,but not through the four hormone ways studied in this research. In the aspect of abiotic stress,OsWRKY7 might participate in drought,high salt and extreme temperature stresses through the ABA way; OsWRKY11 had the possibility to be involved in high salt stress; OsWRKY30 was possible to participate in high salt and hot stresses; OsWRKY70 might be involved in high salt,drought and extreme temperature stresses; OsWRKY89 might be involved in hot stress,but not through the four hormone ways studied in this research. |
| Key words: OsWRKY transcription factor rice blast stress abiotic stress real-time PCR gene expression |
