| 引用本文: | 高慧霞, 梁云慧, 姚妙卓, 刘澄玉, 张鹏飞, 刘亚令.甘草的遗传多样性和谱系地理结构的研究[J].广西植物,2025,45(12):2214-2226.[点击复制] |
| GAO Huixia, LIANG Yunhui, YAO Miaozhuo, LIU Dengyu,
ZHANG Pengfei, LIU Yaling.Genetic diversity and phylogeographic structure of Glycyrrhiza uralensis[J].Guihaia,2025,45(12):2214-2226.[点击复制] |
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| 甘草的遗传多样性和谱系地理结构的研究 |
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高慧霞1, 梁云慧1, 姚妙卓1, 刘澄玉1, 张鹏飞2, 刘亚令1,3*
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1. 山西农业大学 生命科学学院, 山西 太谷 030801;2. 山西农业大学 园艺学院, 山西 太谷 030801;3. 山西农业大学 动物医学学院, 中兽医药现代化山西省重点实验室, 山西 太谷 030801
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| 摘要: |
| 为揭示甘草(Glycyrrhiza uralensis)的遗传多样性和谱系地理结构,该研究基于ITS和3段叶绿体基因(matK、psbA-trnH和trnS-trnG)序列对20个甘草居群100份样本进行扩增后测序,并对其数据进行分析。结果表明:(1)ITS序列长度为691 bp,包含63个变异位点,单倍型多样性为0.265,核苷酸多样性为0.002 69; cpDNA序列长度为1 976 bp,包含740个变异位点,单倍型多样性为0.907,核苷酸多样性为0.026 39。在物种水平上均表现出较高的遗传多样性。(2)分子方差分析结果显示,甘草的遗传分化主要来源于居群内,居群间遗传分化水平较低,基因流(Nm)较高(ITS:Nm=1.349; cpDNA:Nm=1.520)。(3)遗传分化系数(Nst与Gst)比较结果显示,甘草居群不存在明显的谱系地理结构。中性检验结果与失配分析曲线表明甘草居群整体并未经历扩张事件。(4)单倍型地理分布图的分析结果显示,陕西、青海、新疆、内蒙古和甘肃地区甘草的单倍型多样性较高,并且这些地区位于地理分布图中心位置,故推测其可能为甘草在中国西北和华北地区的冰期避难所。该研究利用不同的DNA条形码对甘草的遗传多样性和地理分布格局进行了解析,为今后甘草种质资源的保护与合理利用提供了理论依据。 |
| 关键词: 甘草, ITS序列, cpDNA序列, 地理分布格局, 谱系地理学 |
| DOI：10.11931/guihaia.gxzw202409047 |
| 分类号:Q948 |
| 文章编号:1000-3142(2025)12-2214-13 |
| 基金项目:山西省回国留学人员科研资助项目(2020-069); 国家自然科学基金(32070378); 山西省现代农业产业技术体系建设专项资金(2021-11); 山西农业大学生物育种工程项目(YZGC136)。 |
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| Genetic diversity and phylogeographic structure of Glycyrrhiza uralensis |
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GAO Huixia1, LIANG Yunhui1, YAO Miaozhuo1, LIU Dengyu1,
ZHANG Pengfei2, LIU Yaling1,3*
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1. College of Life Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi, China;2. College of Horticulture, Shanxi Agricultural
University, Taigu 030801, Shanxi, China;3. Shanxi Key Laboratory for Modernization of TCVM, College
of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, Shanxi, China
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| Abstract: |
| To reveal the genetic diversity and phylogeographic structure of Glycyrrhiza uralensis, this study amplified and sequenced 100 samples from 20 populations of G. uralensis based on ITS and three chloroplast gene sequences(matK, psbA-trnH, and trnS-trnG), and analyzed the data. The results were as follows:(1)The length of ITS sequence was 691 bp, containing 63 variable sites, with a haplotype diversity of 0.265 and a nucleotide diversity of 0.002 69; the length of cpDNA sequence was 1 976 bp, containing 740 variable sites, with a haplotype diversity of 0.907 and a nucleotide diversity of 0.026 39. These findings demonstrated high genetic diversity at the species level.(2)Molecular variance analysis result revealed that the genetic differentiation of G. uralensis primarily occurred within populations, with relatively low levels of genetic differentiation between populations and relatively high gene flow(ITS: Nm=1.349; cpDNA: Nm=1.520).(3)The comparison results of genetic differentiation coefficients(Nst and Gst)indicated that there was no significant phylogeographic structure within G. uralensis populations. The results of neutral test and mismatch analysis curve suggested that the overall G. uralensis population did not experience expansion events.(4)Haplotype geographical distribution analysis results revealed that G. uralensis in Shaanxi, Qinghai, Xinjiang, Inner Mongolia and Gansu exhibited relatively high haplotype diversity and was located at the center of the geographical distribution map, suggesting that this region might have served as a glacial refuge of G. uralensis in Northwest and North China. The study analyzes the genetic diversity and geographical distribution patterns of G. uralensis using different DNA barcodes, providing a theoretical basis for the conservation and rational utilization of G. uralensis germplasm resources in the future. |
| Key words: Glycyrrhiza uralensis, ITS sequence, cpDNA sequence, geographical distribution pattern, phylogeography |
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