| 摘要: |
| 花色作为被子植物适应生态环境的关键表型特征,其形成是遗传调控网络与环境因子间协同作用的结果。当前的研究聚焦于花色表型的维持机制、驱动因素及其分子调控网络解析,然而针对土壤环境因子与分子调控网络的互作机制研究仍存在不足。因此,该研究以产生4种颜色花(白色、浅白色、浅紫色及紫色)的二型花柱植物帕米尔报春(Primula pamirica)为研究材料,通过测定花冠色彩参数、根系土壤含水量及矿物质元素含量,并结合其转录组数据分析,初步筛选出参与花色多态性形成的候选转录因子。结果表明:(1)白色和浅白色花冠植株多见于高水分含量-低矿物质元素含量的土壤环境,而紫色和浅紫色花冠植株则主要分布于低水分含量-高矿物质元素含量的土壤环境。(2)苯丙氨酸代谢、类胡萝卜素合成、金属离子转运及氨基酸转运等通路可能在花色形成中发挥重要作用。(3)MYB、bHLH、ZIP和WRKY基因家族的相关成员可能是花色形成的关键候选基因,其中ZIP和WRKY基因家族的相关成员可能在紫色花冠的形成中表现出显著调控效应。综上认为,帕米尔报春花色多态的形成是遗传调控网络与土壤环境因子(土壤水分与矿物质元素)协同作用的结果。 |
| 关键词: 帕米尔报春, 花色多态性, 土壤环境因子, 遗传调控, 转录组学 |
| DOI：10.11931/guihaia.gxzw202502030 |
| 分类号:Q943 |
| 文章编号:1000-3142(2025)12-2271-13 |
| 基金项目:新疆维吾尔自治区自然科学基金面上项目(2022D01A231); 国家自然科学基金(31400279, 32460261); 喀什大学校级创新团队建设项目。 |
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| Effects of different soil conditions on the flower color polymorphism of Primula pamirica and transcriptomic analysis |
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WANG Yibi1, SAILAIJIANG Maierhaba1,2, ABDUSALAM Aysajan 1,2*
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1. College of Life and Geographic Sciences, Kashi University, Kashi 844000, Xinjiang, China;2. Key Laboratory
of Biological Resources and Ecology of Pamirs Plateau in Xinjiang, Kashi 844000, Xinjiang, China
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| Abstract: |
| Flower color, as a key phenotypic characteristic exhibited by angiosperms in their adaptation to the ecological environment, is the result of the synergistic interaction between genetic regulatory networks and environmental factors. Current research primarily focuses on the maintenance mechanisms, driving factors, and molecular regulatory networks of flower color. However, there is still a lack of systematic studies on the interaction mechanisms between soil environmental factors and molecular regulatory networks. This study utilized Primula pamirica, a distylous plant producing flowers in four flower-color morphs(white, light white, light purple, and purple), as the experimental material. A comprehensive comparative analysis was conducted based on the corolla color parameters, soil water contents and mineral element contents of the plant roots, and transcriptomic profiling data across the four flower-color morphs, and preliminary screened to identify candidate transcription factors involved in the formation of flower color polymorphism. The results were as follows:(1)Plants with white and light white corollas predominantly occured in soil environments characterized by high water content and low mineral element content, whereas those with purple and light purple corollas were frequently distributed in soils with low water content and high mineral element content.(2)Pathways such as the phenylalanine metabolism, carotenoid biosynthesis, metal ion transport, and amino acid transport played critical roles in floral pigmentation regulation.(3)Related members of the MYB, bHLH, ZIP, and WRKY gene families were identified as key candidate genes of floral formation, with related members of ZIP and WRKY gene families showing significant regulatory effects on purple corolla formation. In conclusion, the flower color polymorphism in P. pamirica is the result of the genetic regulatory networks and soil environmental factors(soil water status and mineral elements). |
| Key words: Primula pamirica, florwer color polymorphism, soil environmental factors, genetic regulation, transcriptomics |
