| 引用本文: | 刘依婷, 骆冰倩, 王雅兰, 李永宁, 高继海.低温胁迫下兴安白芷与川白芷种子转录组特征及激素调控基因的比对研究[J].广西植物,2025,45(12):2284-2295.[点击复制] |
| LIU Yiting, LUO Bingqian, WANG Yalan, LI Yongning, GAO Jihai.Comparative study on the transcriptome characteristics and hormone regulatory genes of Angelica dahurica and Angelica dahurica cv. ‘Hangbaizhi' seeds under low-temperature stress[J].Guihaia,2025,45(12):2284-2295.[点击复制] |
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| 摘要: |
| 为解析低温胁迫对兴安白芷与川白芷种子萌发的影响,探究不同品种白芷种子的抗寒能力及关键基因,该研究以兴安白芷和川白芷种子的低温和常温萌发对比实验为基础,对不同处理组别进行转录组测序,分析低温胁迫下种子萌发的关键激素脱落酸(ABA)与赤霉素(GA)的差异表达,并进行qRT-PCR验证。结果表明:(1)低温环境下的2个品种白芷种子活力显著下降且兴安白芷种子活力明显高于川白芷。(2)转录组分析显示,低温下Cu/ZnSOD、CYP707A、CAT的表达量显著降低; ABA(PYL)与GA(SNE、GIS)相关基因表达显著升高。(3)对比2个品种,兴安白芷的PsaA、BIP表达量更高,而川白芷的ABI表达更高。(4)低温胁迫下2个白芷品种种子活性下降主要与Cu/ZnSOD、CYP707A和CAT等关键基因表达显著降低相关,并且2种白芷种子的萌发差异主要与ABA和GA相关基因的差异表达有关。综上认为,这些因素的协同作用对种子活性具有重要影响。该研究结果为兴安白芷与川白芷种子抗寒研究提供了新的基因参考。 |
| 关键词: 兴安白芷, 川白芷, 低温胁迫, 植物激素信号转导, 种子萌发, 转录组 |
| DOI：10.11931/guihaia.gxzw202408041 |
| 分类号:Q943 |
| 文章编号:1000-3142(2025)12-2284-12 |
| 基金项目:中央财政重点项目国家中医药跨学科创新团队(ZYYCXTD-D-202209); 成都中医药大学杏林学者项目(QJRC2022029); 特色中药资源多维评价与产品开发创新团队(2022C001); 川产道地药材大品种精深加工关键技术研究与产品开发示范(2020YFN0152)。 |
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| Comparative study on the transcriptome characteristics and hormone regulatory genes of Angelica dahurica and Angelica dahurica cv. ‘Hangbaizhi' seeds under low-temperature stress |
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LIU Yiting, LUO Bingqian, WANG Yalan, LI Yongning, GAO Jihai*
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Key Laboratory of Distinctive Chinese Medicine Resources in Southwest China, School of Pharmacy,
Chengdu University of Chinese Medicine, Chengdu 611137, China
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| Abstract: |
| To analyze the effects of low-temperature stress on seed germination of Angelica dahurica and A. dahurica cv. ‘Hangbaizhi', the cold resistance of seeds from different A. dahurica cultivars and the key genes were explored. Based on the comparative experiment of low-temperature and normal-temperature germination of A. dahurica and A. dahurica cv. ‘Hangbaizhi' seeds, transcriptome sequencing was performed on different treatment groups to analyze the differential expressions of key hormones abscisic acid(ABA)and gibberellins(GA)during seed germination under low-temperature stress. The outcomes were verified by qRT-PCR. The results were as follows:(1)The seed vigor of the two A. dahurica varieties significantly decreased under low-temperature conditions, and the seed vigor of A. dahurica was significantly higher than that of A. dahurica cv. ‘Hangbaizhi'.(2)Transcriptome analysis shows that the expression levels of Cu/ZnSOD, CYP707A, and CAT significantly decreased under low-temperature conditions; while the expressions of ABA(PYL)and GA(SNE, GIS)-related genes significantly increased.(3)In the two varieties, A. dahurica has higher expression levels of PsaA and BIP, while A. dahurica cv. ‘Hangbaizhi' has higher expression of ABI.(4)The decrease in seed activity of two varieties of A. dahurica under low-temperature stress is mainly related to the significant reduction in the expressions of key genes such as Cu/ZnSOD, CYP707A, and CAT; the differences in seed germination between the two A. dahurica varieties are mainly related to the differential expressions of ABA and GA related genes. In conclusion, the synergistic work of these factors ultimately significantly affects seed activity. This study provides new genetic references for the research of cold resistance in seeds of A. dahurica and A. dahurica cv. ‘Hangbaizhi'. |
| Key words: Angelica dahurica, Angelica dahurica cv.‘Hangbaizhi', low temperature stress, plant hormone signal transduction, seed germination, transcriptome |
