| 引用本文: | 袁 媛, 董家帮, 徐艳姣, 李 炎, 张晓玉,
吴 靓, 陈永生, 梁艳丽.太白贝母种子低温萌发过程中C3H基因家族鉴定及表达分析[J].广西植物,2026,46(1):110-122.[点击复制] |
| YUAN Yuan, DONG Jiabang, XU Yanjiao, LI Yan, ZHANG Xiaoyu,
WU Liang, CHEN Yongsheng, LIANG Yanli.Identification and expression analysis of C3H gene family during low-temperature germination of Fritillaria taipaiensis seeds[J].Guihaia,2026,46(1):110-122.[点击复制] |
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| 太白贝母种子低温萌发过程中C3H基因家族鉴定及表达分析 |
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袁 媛1,2, 董家帮1,2, 徐艳姣1,2, 李 炎1,2, 张晓玉1,2,
吴 靓1,2, 陈永生3, 梁艳丽1,2*
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1. 云南农业大学 农学与生物技术学院, 昆明 650201;2. 云南农业大学 西南中药材种质创新与利用国家地方联合工程研究中心/
云南省药用植物生物学重点实验室, 昆明 650201;3. 丽江绿之源生物药业科技开发有限公司, 云南 丽江 674100
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| 摘要: |
| 为解析C3H基因在太白贝母种子萌发过程中的作用,基于低温萌发转录组数据,该研究通过生物信息学的方法对太白贝母C3H基因家族进行系统鉴定及表达分析,筛选出5个低温高表达候选基因并解析其对赤霉素(GA3)和脱落酸(ABA)的响应机制。结果表明:(1)C3H家族65个成员中7个为稳定亲水蛋白、58个不稳定亲水蛋白,蛋白长度差异显著(197～1 451 aa),仅FtC3H43和FtC3H50定位于叶绿体/内质网,其余位于细胞核。(2)保守基序分析发现,Motif1和Motif3普遍存在C3H蛋白中; 系统进化分析结果显示,196条C3H蛋白被聚类为5个不同的分支,分别标记为I-Ⅴ,太白贝母C3H成员与拟南芥和水稻表现出较近的亲缘关系。(3)GA3处理下,FtC3H22和FtC3H35在A-C时期促进萌发,FtC3H40在A期、FtC3H46在E期响应调控; ABA处理时FtC3H46显著上调,而FtC3H22和FtC3H35下调,推测FtC3H46参与ABA介导的抗寒途径,FtC3H22和FtC3H35则正向调控萌发。该研究揭示了C3H蛋白家族在太白贝母种子萌发过程中的重要作用和复杂调控机制。 |
| 关键词: 太白贝母, C3H, 种子萌发, 生物信息分析, 表达模式 |
| DOI：10.11931/guihaia.gxzw202411029 |
| 分类号:Q943 |
| 文章编号:1000-3142(2026)01-0110-13 |
| 基金项目:云南省科技厅重大科技专项(202302AA310030); 云南省科技厅乡村振兴科技专项(202304BI090009); 迪庆藏族自治州科技计划项目(2023ZXND01); 科技人才与平台计划项目(202005AC160040); 云南省科技厅农业联合专项(202101BD070001-008)。 |
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| Identification and expression analysis of C3H gene family during low-temperature germination of Fritillaria taipaiensis seeds |
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YUAN Yuan1,2, DONG Jiabang1,2, XU Yanjiao1,2, LI Yan1,2, ZHANG Xiaoyu1,2,
WU Liang1,2, CHEN Yongsheng3, LIANG Yanli1,2*
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1. College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China;2. National and Local Joint Engineering
Research Center for Germplasm Innovation and Utilization of Southwest Chinese Medicinal Materials/Key Laboratory of Medicinal
Plant Biology in Yunnan Province, Yunnan Agricultural University, Kunming 650201, China;3. Lijiang Lü4.zhiyuan
Biotechnology Pharmaceutical Technology Development Co., Ltd., Lijiang 674100, Yunnan, China
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| Abstract: |
| To analyze the role of C3H genes germination of Fritillaria taipaiensis seeds, this study utilized low-temperature germination transcriptome data and bioinformatics methods to systematically identify and analyze the expression of the C3H gene family in F. taipaiensis. Five cold-inducible high expression candidate genes were identified and characterized to elucidate their regulatory mechanisms in response to gibberellic acid(GA3)and abscisic acid(ABA). The results were as follows:(1)Among 65 identified C3H family members, 7 were classified as stable hydrophilic proteins and 58 exhibited instability, with significant variation in protein length(197-1 451 aa). Only FtC3H43 and FtC3H50 were localized to chloroplast/endoplasmic reticulum, while the majority were nuclear-localized.(2)Conserved motif analysis revealed that Motif1 and Motif3 were widely present in C3H proteins. Phylogenetic analysis results showed that 196 C3H proteins were clustered into five distinct clades(I-V), with F. taipaiensis C3Hs showing close evolutionary relationships to those in Arabidopsis and rice.(3)Under GA3 treatment, FtC3H22 and FtC3H35 promoted germination during early-to-mid stages(A-C), while FtC3H40 and FtC3H46 exhibited stage-specific expression at stages A and E, respectively. ABA treatment significantly upregulated FtC3H46 but downregulated FtC3H22 and FtC3H35, suggesting FtC3H46 might mediate ABA-dependent cold stress responses, whereas FtC3H22 and FtC3H35 played positive roles in germination regulation. This study reveals the important role and complex regulatory mechanism of the C3H protein family in the seed germination of F. taipaiensis. |
| Key words: Fritillaria taipaiensis, C3H, seed germination, bioinformation analysis, expression pattern |
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