| 引用本文: | 王 宇, 陈邦清, 高 晗, 王毅敏, 谭 艳, 姚 溢, 高本旺.濒危植物珙桐根际微生物和根内生菌群落特点及功能分析[J].广西植物,2026,(1):173-185.[点击复制] |
| WANG Yu, CHEN Bangqing, GAO Han, WANG Yimin,
TAN Yan, YAO Yi, GAO Benwang.Characteristics and functional analysis of rhizosphere microorganisms and root endophytes of endangered plant Davidia involucrata[J].Guihaia,2026,(1):173-185.[点击复制] |
|
| |
|
|
| 本文已被：浏览 30次 下载 9次 |
码上扫一扫！ |
|
|
| 濒危植物珙桐根际微生物和根内生菌群落特点及功能分析 |
|
王 宇1, 陈邦清1, 高 晗2, 王毅敏2, 谭 艳1, 姚 溢2, 高本旺1*
|
|
1. 宜昌三峡大老岭自然保护区管理局, 湖北 宜昌 443000;2. 三峡植物园管理处, 湖北 宜昌 443000
|
|
| 摘要: |
| 为研究不同种源珙桐(Davidia involucrata)根际和根内微生物群落特征及功能规律,该研究以珙桐根际土壤和根部组织为研究材料,通过16S rRNA基因和ITS序列扩增子测序技术,对其群落组成、多样性、与理化因子的相关性及共现网络进行了分析且对其功能进行了预测。结果表明:(1)两个种源根际和根内微生物群落之间α多样性差异不显著,但β多样性存在显著性差异。(2)两个种源珙桐根际和根内优势细菌为变形菌门、酸杆菌门和放线菌门,而真菌则以子囊菌门与担子菌门为主; 属分类水平则表现出特异性富集。(3)共现网络分析揭示了珙桐根际与根内细菌互作复杂度更高,其网络平均度及模块化等网络拓扑指数均高于真菌,揭示了真菌群落更高的保守性。(4)LEfSe分析表明,存在39个细菌分类单元和30个真菌分类单元且种源间具有显著性差异。(5)理化因子与微生物群落相关性显示,仅土壤有机碳与根际土壤细菌优势群落呈显著正相关,土壤速效钾与根内真菌群落呈显著正相关,其他理化因子与微生物群落无显著相关性。(6)功能预测表明,根际和根内优势细菌主要参与新陈代谢等功能,真菌则以腐生营养型为主。两个种源根际细菌群落在次生代谢物的合成、转录,以及折叠、分选和降解等11种功能均存在显著性差异,根际真菌仅地衣共生真菌功能存在显著性差异。综上认为,种源可影响微生物的组成与互作,并塑造功能差异化,这些差异可能影响珙桐的生态适应性,因此该研究结果可为濒危植物珙桐的迁地保护提供微生物组层面的理论支撑。 |
| 关键词: 珙桐, 根际, 内生菌, 群落结构, 共现网络, 功能预测 |
| DOI：10.11931/guihaia.gxzw202502036 |
| 分类号:Q948 |
| 文章编号:1000-3142(2026)01-0173-13 |
| 基金项目:2023年中央林业草原生态保护恢复项目(10000017Z175070050001); 2024年湖北省林业生态文明建设项目(第三批)(42000021816T300000130)。 |
|
| Characteristics and functional analysis of rhizosphere microorganisms and root endophytes of endangered plant Davidia involucrata |
|
WANG Yu1, CHEN Bangqing1, GAO Han2, WANG Yimin2,
TAN Yan1, YAO Yi2, GAO Benwang1*
|
|
1. Dalaoling Nature Reserve Administration of Yichang Three Gorges, Yichang 443000, Hubei, China;2. Three Gorges Botanical Garden Management Office, Yichang 443000, Hubei, China
|
| Abstract: |
| To investigate the characteristics and functional patterns of rhizosphere and root endophytic microbial communities in different provenances of Davidia involucrata, this study took rhizosphere soil and root tissues as research materials, systematically examined community composition, diversity, physicochemical factor correlations, co-occurrence networks, and curried out the function prediction through 16S rRNA gene and ITS sequence amplicon sequencing technology. The results were as follows:(1)No significant α diversity differences were observed, but distinct β diversity patterns existed between provenances across compartments.(2)The dominant bacterial phyla included Proteobacteria, Acidobacteriota, and Actinobacteriota, while the dominant fungal phyla were Ascomycota and Basidiomycota; genus-level analysis revealed provenance-specific enrichment patterns.(3)Co-occurrence network analysis revealed bacterial communities exhibited greater interaction complexity compared to fungi, as evidenced by a higher average degree and modularity in network topology, reflecting the greater ecological conservatism of fungal communities.(4)LEfSe analysis identified 39 bacterial and 30 fungal taxa that demonstrated significant provenance-driven differentiation.(5)Physicochemical factor and microbial community correlation analysis indicated that soil organic carbon significantly positively correlated with the dominant bacterial communities in rhizosphere soil, while soil available potassium was significantly positively correlated with endophytic fungal communities within roots, with no other physicochemical factor and microbial community correlations observed.(6)Functional prediction indicated that dominant bacteria in the rhizosphere and roots endosphere primarily engage in functions such as metabolism, while fungi were predominantly saprotrophic. The rhizosphere bacterial communities from the two provenances exhibited significant differences in 11 functions, including the biosynthesis of other secondary metabolites, transcription, folding, sorting and degradation. For rhizosphere fungi, only the function of lichen symbiotic fungi showed significant differences. In conclusion, provenance can affect the composition and interactions of microorganisms, and shape differentiated functions which may influence the ecological adaptability of D. involucrata, therefore, the findings of this study can provide theoretical support at the microbiome level for the ex-situ conservation of the endangered plant D. involucrata. |
| Key words: Davidia involucrata, rhizosphere, endophytes, community structure, co-occurrence network, functional prediction |
|
|
|
|
|
