| 引用本文: | 安常蓉, 李 芸, 崇慧影, 文光琴, 段如雁.内生真菌接种方式对蓝莓幼苗生长及生理特性的影响[J].广西植物,2026,46(6):919-930.[点击复制] |
| AN Changrong, LI Yun, CHONG Huiying, WEN Guangqin, DUAN Ruyan1*[点击复制] |
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| 内生真菌接种方式对蓝莓幼苗生长及生理特性的影响 |
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安常蓉1, 李 芸2, 崇慧影2, 文光琴2, 段如雁1*
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1. 贵州省生物研究所, 贵阳 550009; 2. 贵州省植物园, 贵阳 550004
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| 摘要: |
| 为探讨蓝莓接种内生真菌的有效方法,该研究以1年生蓝莓组培苗和1株内生真菌(Talaromyces aculeatus)为材料,设计了根系与菌悬液浸泡(F1)、菌悬液与基质混合(F2)、挑取固体菌块(F3)、修剪根系后与菌悬液浸泡(F4)和菌悬液浇灌(F5)5种接种方式进行盆栽试验,以无菌水浸泡及不接种内生真菌的盆栽苗为对照(CK),观察接种后幼苗根系侵染情况,测定幼苗生长生理指标。结果表明:(1)F2处理的蓝莓幼苗根系侵染率最高,其次是F5处理,5种接种方式处理的根系侵染率分别是CK的2.6倍、3.7倍、3.4倍、3.2倍和3.6倍。(2)除叶生物量和根生物量指标以外,接种菌根的蓝莓幼苗生长指标整体高于CK,与CK相比,5种接种方式处理下的苗高生长量、地径生长量和总生物量增幅分别为3.21%～30.47%、16.37%～37.43%和9.69%～39.79%; 总根长、总根表面积、总根体积和根尖数的增幅分别为38.63%～118.43%、5.08%～94.89%、11.97%～65.14%和28.90%～92.44%。(3)不同接种方式处理的蓝莓幼苗生理特性差异明显,F3处理的叶片最大电子传递速率、最小饱和光强、叶绿素b含量和叶绿素总量较高,F2处理的叶绿素a含量和根系活力较高,CK处理的初始斜率高于接种处理。(4)通过隶属函数法对各接种方式处理进行综合评价,排名依次为F3>F2>F5>F4>F1>CK。综上认为,接种方式显著影响蓝莓内生真菌的促生效果,适宜接种方式为固体菌块、菌悬液与基质混合以及菌悬液浇灌接种,为蓝莓菌根苗高效栽培提供重要参考。 |
| 关键词: 内生真菌, 蓝莓幼苗, 接种方式, 侵染率, 促生效应, 隶属函数 |
| DOI：10.11931/guihaia.gxzw202505008 |
| 分类号:Q945 |
| 文章编号:1000-3142(2026)06-0919-12 |
| 基金项目:财政部和农业农村部 国家现代农业产业技术体系项目(CARS-29-24); 贵州省科技重大专项(黔科合重大专项字[2024]028号); 贵州省科技计划项目(黔科合支撑[2019]2267号、[2020]4Y012号)。 |
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| Effects of inoculation methods of endophytic fungi on growth and physiological characteristics of blueberry seedlings |
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AN Changrong1, LI Yun2, CHONG Huiying2, WEN Guangqin2, DUAN Ruyan1*
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1. Guizhou Institute of Biology, Guiyang 550009, China; 2. Guizhou Botanical Garden, Guiyang 550004, China
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| Abstract: |
| To explore effective methods for inoculating blueberry with endophytic fungi, a pot experiment was conducted using one-year-old tissue-cultured blueberry seedlings and a strain of endophytic fungus(Talaromyces aculeatus). Five inoculation methods were designed, as root immersion in fungal suspension(F1), mixing fungal suspension with substrate(F2), inoculating with solid fungal blocks(F3), root immersion in fungal suspension after root pruning(F4), and irrigation with fungal suspension(F5). Seedlings immersed in sterile water without inoculation served as the control(CK). Root infection situation after inoculation was statistically analyzed, and determined some indexes that relate to growth and physiology. The results were as follow:(1)The root infection rates of blueberry were significantly different among the inoculation methods, the seedlings inoculated by F2 showed a higher root infection rate than those by other methods, and the root infection rate by F5 was the second highest. The root infection rates of treatments F1, F2, F3, F4 and F5 were 2.6, 3.7, 3.4, 3.2 and 3.6 times CK, respectively.(2)Except for leaf biomass and root biomass, all other growth parameters of blueberry seedlings inoculated with endophytic fungi were significantly higher than those of CK. Compared to the CK, the height increment, ground diameter increment, and total biomass of seedlings treated by five inoculation methods increased by 3.21%-30.47%, 16.37%-37.43%, and 9.69%-39.79%, respectively. The total root length, total root surface area, total root volume and root tip number increased by 38.63%-118.43%, 5.08%-94.89%, 11.97%-65.14%, and 28.90%-92.44%.(3)The physiological characteristic of blueberry seedlings by different inoculation methods showed significant differences, the maximum electron transport rate, minimum saturated light intensity, chlorophyll b content, and total chlorophyll content under the F3 were higher than other treatments, the chlorophyll a content and root activity showed higher with F2, while the CK had a higher initial slope than inoculation with endophytic fungi.(4)Based on the membership function analysis, the comprehensive evaluation result was F3 > F2 > F5 > F4 > F1 > CK. The inoculation method significantly affects the impact on the growth-promoting effect of endophytic fungi. The suitable inoculation methods are solid fungal blocks, mixing fungal suspension with substrate, and fungal suspension irrigation, which provide important insights for the efficient cultivation of endophytic blueberry seedlings. |
| Key words: endophytic fungi, blueberry seedlings, inoculation methods, infection rate, growth-promotion effect, membership function |
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