| 摘要: |
| 为研究罗汉果苷IIE在脂多糖(LPS)和棕榈酸(PA)联合诱导小鼠巨噬细胞糖尿病炎症模型中的作用,该研究采用1 ng·μL-1的LPS和100 μmol·L-1的PA联合处理小鼠巨噬细胞RAW 264.7构建糖尿病炎症模型,并运用qRT-PCR分别检测0、1、3、6、12、24 h六个不同时间点细胞中炎症因子TNF-α mRNA的表达水平变化。结果表明:(1)1 ng·μL-1的LPS和100 μmol·L-1 的PA两者共同处理小鼠巨噬细胞RAW 264.7在24 h时产生的炎症协同作用最好;(2)在LPS(1 ng·μL-1)和PA(100 μmol·μL-1)联合诱导小鼠巨噬细胞12 h后,进而用20 μmol·L-1的罗汉果苷IIE处理12 h,qRT-PCR检测结果发现,20 μmol·L-1的罗汉果苷IIE处理12 h可显著降低LPS和PA联合诱导的细胞内炎症因子TNF-α mRNA表达水平,说明罗汉果苷IIE能够有效降低巨噬细胞的炎症水平。综上认为,罗汉果苷IIE可能在治疗糖尿病中起到一定的作用,该研究结果为糖尿病治疗新策略的提出提供了参考。 |
| 关键词: 糖尿病炎症模型, 罗汉果苷IIE, 脂多糖, 棕榈酸, 小鼠巨噬细胞 |
| DOI：10.11931/guihaia.gxzw202203051 |
| 分类号:Q946 |
| 文章编号:1000-3142(2022)09-1454-05 |
| 基金项目:广西高校中青年教师科研基础能力提升项目(2020KY12019)[Supported by Project of Young and Middle-Aged Teachers Basic Ability Promotion for Scientific and Technological Research of College and Universities in Guangxi(2020KY12019)]。 |
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| Mogroside IIE effects on macrophage in diabetic inflammatory models |
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HUANG Kai1, XIAO Juan1, LI Aili2*
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1. Guangxi Laboratory Molecular Medicine in Liver Injury and Repair, Affiliated Hospital of Guilin Medical University,
Guilin 541001, Guangxi, China;2. Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi, China
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| Abstract: |
| To investigate the role of mogroside IIE in the combination of lipopolysaccharide(LPS)and palmitic acid(PA)in the induction of diabetic inflammation in mouse macrophages, we used 1 ng· μl-1 LPS and 100 μmol·l-1 PA to establish a diabetic inflammation model of mouse macrophages RAW 264.7, and used qRT-PCR to detect the expression of inflammatory factor TNF-α mRNA in cells at 0, 1, 3, 6, 12, 24 h. The results were as follows:(1)The synergistic effect of LPS(1 ng· μL-1)and PA(100 μmol· L-1)on macrophage RAW 264.7 was the best at 24 h.(2)After mouse macrophages were induced with 1 ng·μL-1 LPS and 100 μmol·L-1 PA for 12 h, and treated with 20 μmol·L-1 mogroside IIE for 12 h, qRT-PCR result showed that 20 μmol·L-1 mogroside IIE treatment for 12 h could significantly reduce the expression of inflammatory factor TNF-α mRNA induced by LPS and PA, indicating that mogroside IIE can effectively reduce the inflammation level of macrophages. In conclusion, mogroside IIE may play a certain role in the treatment of diabetes, which provides a reference for the proposal of new strategies for the treatment of diabetes. |
| Key words: inflammation model of diabetes, mogroside IIE, lipopolysaccharide, palmitic acid, mouse macrophages |
