软枣猕猴桃‘天源红'离体再生体系的建立

Establishment of regeneration of Actinidia arguta ‘Tianyuanhong'

关键词:  全红型软枣猕猴桃, 组织培养, 愈伤组织, 丛生芽

Key words:  all-red Actinidia arguta, tissue culture, callus, multiple shoot clump

摘要:

为了建立快速高效的全红型软枣猕猴桃离体再生体系,该研究以果皮、果肉均为红色的软枣猕猴桃新品种‘天源红'(Actinidia arguta)带腋芽茎段和幼嫩叶片、叶柄为外植体材料,采用组织培养的方法,研究适合其离体再生的外植体类型以及最佳植物生长物质组合。结果表明:初春带腋芽的茎段是最好的获得无菌苗的外植体材料,诱导腋芽出芽的最佳植物生长物质组合为MS+6-BA 0.5 mg·L^-1+IBA 1.0 mg·L^-1; 研究发现叶柄比叶片更适合进行‘天源红'愈伤组织诱导,叶柄诱导的最佳植物生长物质组合为MS+ZT 0.5 mg·L^-1; 同时,研究了不定芽增殖的最佳植物生长物质组合为MS+ZT 1.0 mg·L^-1; 此外使用6-BA也可以达到较高的不定芽增殖率,在生产上可以替代ZT进行不定芽分化,即MS+6-BA 2.0 mg·L^-1+IBA 0.5 mg·L^-1; 较适宜的生根培养基为1/2 MS+NAA 0.2 mg·L^-1; 生根后的组培苗在珍珠岩:泥炭:细沙=1:1:1的基质配比中能够达到98%的移栽成活率。该研究结果建立了全红型软枣猕猴桃的离体再生体系,为全红型软枣猕猴桃苗木快繁、工厂化育苗提供了技术支持,同时建立的再生体系为软枣猕猴桃遗传转化研究提供了基础。

Abstract:

‘Tianyuanhong' was a new Actinidia arguta cultivar, with all red pericarp and pulp. In order to establish the rapid and efficient propagation system in vitro in ‘Tianyuanhong', we used stem with axillary bud, young leaf and leaf stem of ‘Tianyuanhong' as the explants, and used tissue culture methods to study explant types and the best plant growth regulator substance combination in this cultivar. The result showed that stem with axillary bud that sprout in early spring was the best explants, and MS+6-BA 0.5 mg·L^-1+IBA 1.0 mg·L^-1 was the suitable plant growth substances combination; leaf stem was the better experimental material than leaf to induce callus, MS+ZT 0.5 mg·L^-1 was the optimizing plant growth substances combination; the best plant growth substances combination in adventitious bud propagation was MS+ZT 1.0 mg·L^-1; and 6-BA could have the same effect in adventitious bud propagation, and could replace ZT for adventitious bud propagation in production, MS+6-BA 2.0 mg·L^-1+IBA 0.5 mg·L^-1was well; 1/2 MS+NAA 0.2 mg·L^-1was the best plant growth substances combination for rooting. After the seedlings rooted, transplanted the seedlings to the substrate, the seedlings could reach the 98% survival rate in the substrate with the ratio of perlite: peat soil: sand=1:1:1. Through this study, the in vitro regeneration system was established in all-red A. arguta ‘Tianyuanhong', which provides a good technical support for seedling micropropagation and factory nursery, at the same time, the established regeneration system provides the basis for the research of genetic transformation in all-red A.arguta.