濒危特有种掌叶木的微卫星遗传多样性研究

滕婕华<sup>1</sup>; 卫家贤<sup>1</sup>; 李象钦<sup>2</sup>; 李超群<sup>3</sup>; 倪 敏<sup>1</sup>; 翁乐逸<sup>1</sup>; 谢国文<sup>1*</sup>

引用本文:	滕婕华, 卫家贤, 李象钦, 李超群, 倪敏, 翁乐逸, 谢国文.濒危特有种掌叶木的微卫星遗传多样性研究[J].广西植物,2017,37(11):1471-1479.[点击复制]
	TENG Jie-Hua, WEI Jia-Xian, LI Xiang-Qin, LI Chao-Qun, NI Min, WENG Le-Yi, XIE Guo-Wen.Endangered species endemic to microsatellite genetic diversity study on Handeliodendron bodinieri [J].Guihaia,2017,37(11):1471-1479.[点击复制]

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濒危特有种掌叶木的微卫星遗传多样性研究
滕婕华¹, 卫家贤¹, 李象钦², 李超群³, 倪敏¹, 翁乐逸¹, 谢国文^1*
1. 广州大学生命科学学院, 广州 510006;2. 广西壮族自治区中国科学院广西植物研究所, 广西桂林 541006;3. 中国科学院植物研究所, 北京 100093

摘要:

掌叶木居群具有较丰富的遗传多样性,该研究利用9对微卫星(SSR)分子标记揭示了掌叶木(Handeliodendron bodinieri)的遗传多样性。结果表明:观测等位基因数(Na)平均为3.903,有效等位基因数(Ne)平均为2.545,期望杂合度(He)平均为0.521,Shannon's多态性信息指数(I)为0.962,PIC平均值为0.465。掌叶木的自然分布居群有相对较高的遗传多样性,但由于人为破坏等因素导致该群体濒危,而濒危并不是因为遗传多样性降低而造成的。居群间的遗传分化为掌叶木8个居群间的遗传一致度为(GI=0.849～0.970),遗传距离为(GD=0.032～0.164)。基于Nei's遗传距离用UPGMA法对掌叶木居群进行聚类,Nei's的基因分化系数为(G_st)为0.027,平均Nei标准遗传分化系数(G'_stN)为0.031,平均Herick's标准遗传分化系数(G'_stH)为0.064,基因流(N_m)为3.368。AMOVA分析结果表明:掌叶木居群间变异占3%,居群内变异占97%,居群内的遗传分化大于居群间的分化。利用Mantel检测发现,居群间的遗传距离与地理距离显著正相关(r=0.299,P<0.05)。该研究结果为掌叶木生物多样性和资源保护与利用提供了更充分的科学依据。

关键词: 掌叶木, 微卫星(SSR), 濒危植物, 遗传多样性, 毛细管电泳

DOI：10.11931/guihaia.gxzw201612036

分类号:Q949.9

文章编号:1000-3142(2017)11-1471-09

基金项目:国家自然科学基金(31270259, 31540069); 全国大学生“挑战杯”课外科技活动项目; 大学生创新训练项目(CX2016010)[Supported by the National Natural Science Foundation of China(31270259,31540069); National University of “Challenge Cup” in Extracurricular Scientific and Technological Activites; Innovation Training Program of University Students(CX2016010)]。

Endangered species endemic to microsatellite genetic diversity study on Handeliodendron bodinieri

TENG Jie-Hua¹, WEI Jia-Xian¹, LI Xiang-Qin², LI Chao-Qun³, NI Min¹, WENG Le-Yi¹, XIE Guo-Wen^1*

1. College of Life Sciences, Guangzhou University, Guangzhou 510006, China;2. Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China;3. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 1. College of Life Sciences, Guangzhou University, Guangzhou 510006, China; 2. Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China; 3. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Abstract:

Genetic diversity of Handeliodendron bodinieri was studied with microsatellite(SSR)markers. We used nine pairs polymorphic microsatellite loci to reveal that H. bodinieri was rich in genetic diversity. Average number of alleles(Na)and effective number of alleles(Ne)were 3.903 and 2.545 respectively. The mean expected heterozygosity(He)was 0.521 and Shannon's diversity(I)was 0.962 and PIC=0.465. Natural populations of H. bodinieri had relatively high level of genetic diversity, however the genetic diversity of the populations were reduced due to factors like sabotage. The majority of genetic variation occurred within populations. The genetic distance(GD)and genetic identity(GI)among eight populations of H. bodinieri were 0.032-0.164, 0.849-0.970, respectively. According to genetic distance UPGMA, genetic differentiation(G_st = 0.027), G'_stN=0.031, G'_stH=0.064 and gene flow(N_m)was 3.368. The results analyzed by AMOVA showed that the variation among the populations was 3%, while the variation within the populations was 97%. Mantel test revealed that there was positive correlation between genetic distance and geographical distance(r=0.299, P<0.05)among the populations. The results are helpful to develop scienfific and valid strategies for protecting the biodiversity of H. bodinieri.

Key words: Handeliodendron bodinieri, microsatellite(SSR), endangered plant, genetic diversity, capillary electrophoresis