核桃青皮的化学成分研究——酚类化合物

魏 欢<sup>1; 2</sup>; 杨建文<sup>1</sup>; 颜小捷<sup>2*</sup>; 李典鹏<sup>2</sup>; 张灵志<sup>3</sup>

引用本文:	魏欢, 杨建文, 颜小捷, 李典鹏, 张灵志.核桃青皮的化学成分研究——酚类化合物[J].广西植物,2018,38(4):463-468.[点击复制]
	WEI Huan, YANG Jianwen, YAN Xiaojie, LI Dianpeng, ZHANG Lingzhi.Chemical constituents from walnut green husk: Phenols[J].Guihaia,2018,38(4):463-468.[点击复制]

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核桃青皮的化学成分研究——酚类化合物
魏欢^1,2, 杨建文¹, 颜小捷^2*, 李典鹏², 张灵志³
1. 桂林理工大学, 广西桂林 541006;2. 广西植物功能物质研究与利用重点实验室, 广西壮族自治区中国科学院广西植物研究所, 广西桂林 541006;3. 中国科学院广州能源研究所, 广州 510640

摘要:

核桃青皮是一种传统的中药材,含有大量的酚类化合物,具有镇痛、消炎、抑菌、抗肿瘤等功效。为了从核桃青皮中分离得到更多的酚类成分,以利于更好地阐明其作用机理,该研究采用大孔树脂DiaionHP-20SS、凝胶SephadexLH-20,HPLC等方法对核桃青皮80%的乙醇提取物进行分离纯化。结果表明:共分离了10个单体化合物,它们的结构经质谱(MS)、一维核磁共振谱(¹H NMR 和¹³C NMR)、二维核磁共振谱(HSQC, HMBC)数据的分析及文献数据的比较确定为没食子酸(1),没食子酸甲酯(2),对羟基苯甲酸(3),3,4-二羟基苯甲酸甲酯(4),6-O-咖啡酸-D-葡萄糖(5),6-O-没食子酸-葡萄糖苷(6),4,8-二羟基-1-四氢萘醌(7),5,8-二羟基-4-甲氧基-1-四氢萘酮(8),5,8-二羟基-1-四氢萘酮(9),4-羟基-1-四氢萘酮(10)。其中,化合物5,化合物6为属内首次分离到。该研究结果为进一步深入研究核桃青皮的化学成分和药理作用提供了一定参考。

关键词: 核桃青皮, 化学成分, 结构鉴定

DOI：10.11931/guihaia.gxzw201703009

分类号:Q946

文章编号:1000-3142(2018)04-0463-06

基金项目:广西壮族自治区八桂学者专项项目(第三批); 广西植物研究所基本业务费(桂置业15011); 广西植物功能物质研究与利用重点实验室主任基金(ZRJJ2015-14)[Supported by the Bagui Scholar Program of the Guangxi Institute of Botany, Guilin(The third batch); Fundamental Research Fund of Guangxi Institute of Botany(15011 ); the Director of Capital Program of Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization(ZRJJ2015-14)]。

Chemical constituents from walnut green husk: Phenols

WEI Huan^1,2, YANG Jianwen¹, YAN Xiaojie^2*, LI Dianpeng², ZHANG Lingzhi³

1. Guilin University of Technology, Guilin 541006, Guangxi, China;2. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guangxi Institute of Botany, Guilin 541006, Guangxi, China;3. Guangzhou Institute of Energy, Chinese Academy of Sciences, Guangzhou 510640, China 1. Guilin University of Technology, Guilin 541006, Guangxi, China; 2. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guangxi Institute of Botany, Guilin 541006, Guangxi, China; 3. Guangzhou Institute of Energy, Chinese Academy of Sciences, Guangzhou 510640, China

Abstract:

Walnut green husk, a traditional Chinese herbal medicine, is often used for analgesic, anti-inflammatory, antibacterial, anti-tumor and so on. Previous studies show that walnut green husk contains lots of phenolic compounds. In order to get more monomer compounds and to facilitate better study of its physiological mechanism, 80% ethanol extract of walnut green husk was separated by the chromatography(macroporous resin HP-20SS, Sepadexs LH-20, and HPLC)and ten compounds were obtained. Their structures were identified by MS,1D(¹H and ¹³C)NMR, 2D(HSQC, HMBC)NMR spectral data analyses, as well as comparison with reported data as:gallic acid(1), methyl gallate(2), p-hydroxybenzoic acid(3), methyl-3,4-dihydroxybenzoate(4), 6-O-caffeic acid-D-glucose(5), 6-O-gallicacid-glucoside(6), 4,8-dihydroxy(7), 4,5,8-trihydroxy-1-tetralone(8), 5,8-dihydroxy-1-tetralone(9), 4-hydroxy-1-tetralone(10). Compounds 5 and 6 were isolated from the genus Juglans for the first time. The results can provide a reference for further study on the chemical composition and pharmacological effects of walnut green husk.

Key words: walnut green husk, constituents, structure identification