‘东红'猕猴桃高效再生体系的建立

Regeneration system of tissue culture of Actinidia chinensis ‘Donghong'

关键词:  猕猴桃, ‘东红', 再生体系, 叶柄, 移栽

Key words:  kiwifruit, Actinidia chinensis ‘Donghong', regeneration system, petiole, transplanting

摘要:

为有力推动猕猴桃产业化种苗生产及推广,快速高效地繁育猕猴桃新种质资源,同时为猕猴桃多倍体育种、转基因育种等新兴育种技术创造新种质资源奠定基础,该研究以‘东红'猕猴桃叶片、叶柄为外植体,探讨了不同植物生长调节剂种类及质量浓度组合对不定芽诱导过程中不定芽形成的影响,并研究了不同植物生长调节剂对‘东红'组培苗不定根诱导的影响。结果表明:‘东红'再生最佳外植体为叶柄,叶柄不定芽再生最佳培养基为MS + 0.5 μg·mL^-1 6-BA + 0.2 μg·mL^-1 NAA,不定芽平均再生率为91.2%; 不定芽经过壮苗培养(MS + 0.2 μg·mL^-1 6-BA + 0.05 μg·mL^-1 NAA),取2～3 cm高幼苗进行生根诱导,不定根再生率为93%,平均根数为6条; 生根后,种苗移栽成活率在80%以上。初步建立了‘东红'叶柄高效再生体系,为猕猴桃快速的产业化种苗生产及推广提供了有力保证,也为后期猕猴桃育种研究提供理论依据。

Abstract:

In order to promote the massive seedling industrialization and popularization of Actinidia chinensis, and multiply the new seedling resources fast and effectively, and lay foundation for the genetic transformation of kiwifruit, the petioles and leaves of A. chinensis ‘Donghong' were chosen as explants, and the effects of different plant growth regulators on frequencies of callus formation and adventitious bud regeneration were investigated by culturing on the induction medium. Moreover, the influence of different types of plant growth regulators on the induction of adventitious roots was also analyzed. The results showed that the combination of different concentrations of 6-BA and NAA had strong effect on the bud regeneration rate, and the optimum combination(MS + 0.5 μg·mL^-1 6-BA + 0.2 μg·mL^-1 NAA)were obtained, in which the frequency of bud regeneration from leaves and petioles reached 63.4% and 91.2% after 35 d culture, respectively. The adventitious bud regeneration rate of petiole explant of A. chinensis ‘Donghong' was significantly higher than that of leaf explant. The frequency of adventitious bud regeneration from petioles was up to 91.2% and the average amount of regenerated buds for each petiole explant was 9-12. The result suggested that IBA was much more effective than NAA for rooting. The best cencentration of IBA was 0.5 μg·mL^-1, and the rate of adventitious root regeneration and the average number of roots per shoot were 93% and 6, respectively. More than 80% of plantlets survived after transplanting. This fast and direct diffe-rentiation simplified the process of adventitious bud formation differentiated from callus, which shortened the culture process and provided a powerful guarantee for the rapid industrialization of seedling production.