ESI-Q-TOF-MS直接进样法比较陕西五个不同地区丹参的化学成分差异(英文)

张亦琳<sup>1*</sup>; 潘 高<sup>1</sup>; 延 永<sup>1</sup>; 葛 颖<sup>2</sup>; 王 茜<sup>1</sup>

引用本文:	张亦琳, 潘高, 延永, 葛颖, 王茜.ESI-Q-TOF-MS直接进样法比较陕西五个不同地区丹参的化学成分差异(英文)[J].广西植物,2019,39(4):490-498.[点击复制]
	ZHANG Yilin, PAN Gao, YAN Yong, GE Ying, WANG Xi.Comparison of chemical constituent in Salvia miltiorrhiza from five different regions in Shaanxi Province by direct injection ESI-Q-TOF-MS[J].Guihaia,2019,39(4):490-498.[点击复制]

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ESI-Q-TOF-MS直接进样法比较陕西五个不同地区丹参的化学成分差异(英文)
张亦琳^1*, 潘高¹, 延永¹, 葛颖², 王茜¹
1. 商洛学院生物医药与食品工程学院, 陕西商洛 726000;2. 西北大学化学与材料科学学院, 合成与天然功能分子化学教育部重点实验室, 西安 710069

摘要:

该研究采用ESI-Q-TOF-MS直接进样分析法对陕西商州、洛南、大荔、丹凤和铜川等五个地区丹参的化学成分进行了比较分析,通过丹参水溶性和脂溶性成分的质谱丰度差异评价了不同地区丹参化学成分含量的变化,对不同产地丹参的化学成分进行了鉴定,综合分析选出了最优的丹参种植产地。结果表明:五地丹参均含有丹参酮ⅡA、隐丹参酮、丹参酮Ⅰ、二氢丹参酮、丹参酚酮、次丹参醌、丹参醌Ⅱ等9种脂溶性化学成分和丹参素、咖啡酸、阿魏酸、迷迭香酸、原紫草酸、紫草酸、原儿茶醛、丹酚酸A、丹酚酸B等9种水溶性化学成分,其中丹参素钠、紫草酸、丹酚酸B和隐丹参酮的含量普遍较高,质谱丰度均大于30%。但是,随着种植环境不同,不同地区丹参的化学成分含量差异很大,如商州产丹参中含有较多具有生物活性的丹参酮类物质,其中丹参酮Ⅰ含量远远高于其他四个产地,质谱丰度达到72.6%,而其他地区丹参酮Ⅰ的质谱丰度为1.8%～11.3%。这表明丹参质量按照地区排序为商州>铜川>大荔>洛南>丹凤。该方法为中药药材质量评价提供了科学、可靠、便捷的途径,为药材规格等级的制定提供了新途径,为陕西丹参种植区域的选择提供了重要信息。

关键词: 丹参, ESI-Q-TOF-MS, 化学成分, 丰度, 药材质量

DOI：10.11931/guihaia.gxzw201804017

分类号:Q946.83

文章编号:1000-3142(2019)04-0490-09

基金项目:国家自然科学基金青年科学基金(21703135); 商洛学院自然科学基金(16SKY021); 国家大学生创新创业训练项目(201711396012)[Supported by the National Natural Science Foundation for Youth of China(21703135); Natural Science Foundation of Shangluo University(16SKY021); the National Student's Innovation and Entrepreneurship Training Program(201711396012)]。

Comparison of chemical constituent in Salvia miltiorrhiza from five different regions in Shaanxi Province by direct injection ESI-Q-TOF-MS

ZHANG Yilin^1*, PAN Gao¹, YAN Yong¹, GE Ying², WANG Xi¹

1. College of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo 726000, Shaanxi, China;2. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, China 1. College of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo 726000, Shaanxi, China; 2. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, China

Abstract:

An direct injection ESI-Q-TOF-MS analysis method was developed to value the chemical constituent in Salvia miltiorrhiza(Danshen)coming from Shangzhou, Luonan, Dali, Danfeng and Tongchuan in Shaanxi Province, through analyzing the MS abundance of water soluble and lipid soluble extracts and obvious variation in chemical constituent. The comparison among different abundances was beneficial to select the optimum planting area of Danshen. The results showed that all Danshen samples from five different regions had nine kinds of lipid soluble chemical constituent(Tanshinone I, Tanshinone ⅡA, Tanshinone ⅡB, Cryptotanshinone, Dihydrotanshinone, Danshenxinkun A, Danshenxinkun D, 2-Isopropyl-8-methylphenanthrene-3,4-dione and 7-beta-hydroxy-8-13- abietadiene-11,12-dione)and nine kinds of water soluble chemical constituent(danshensu, caffeic acid, ferulic acid, rosmarinic acid, prolithospermic acid, lithospermic acid, protocatechuic acid, salvianolic acid A and salvianolic acid B). The contents of danshensu, lithospermic acid, salvianolic acid B and cryptotanshinone were higher than other chemical constituent with a MS abundance of 30% at least. However, with the change of planting environment, the content of the same chemical constituents in Danshen varied greatly in different regions. More tanshinones with important biological activity existed in Danshen coming from Shangzhou, especially the MS abundance of Tanshinone I was as high as 72.6%, which was much higher than that in other four regions ranging from 1.8% to 11.3%. According to the comprehensive comparison, the quality of Danshen was ranged by Shangzhou > Tongchuan > Dali> Luonan> Danfeng. The direct injection ESI-Q-TOF-MS method was not easily affected by the growth environment and extraction conditions of the medicinal materials. It provides a scientific, reliable and convenient way to evaluate the medicine quality and a new way to formulate the specifications and grades of traditional Chinese medicine. The system is suitable for the chemical constituent of Danshen in Shaanxi Province.

Key words: Salvia miltiorrhiza(Danshen), ESI-Q-TOF-MS, chemical constituent, abundance, medicine quality