| 摘要: |
| 为深入研究春兰(Cymbidium goeringii)与春兰奇花品种花器官发育调控的分子机制,该研究采用同源克隆的方法,分别从普通的春兰与春兰奇花品种‘天彭牡丹'的花芽中克隆得到1个cDNA长849 bp D类MADS-box基因CygoSTK(Genbank 登录号为MH917912.1)。结果表明:该基因序列在两种春兰中高度一致,包含1个长705 bp的完整ORF,编码1个由234个氨基酸残基组成的STK进化系MADS-box转录因子; 结构分析表明,CygoSTK转录因子包含1个高度保守的MADS结构域(MADS domain)(1～57)和1个次级保守的K结构域(91～172),其C末端的转录激活区含有两个高度保守的基序,即AGI基序和AGⅡ基序; 进一步用qPCR检测CygoSTK基因在普通的春兰与春兰奇花品种‘天彭牡丹'不同花器官中的相对表达量发现,CygoSTK基因在普通的春兰和春兰‘天彭牡丹'子房中的表达量最高,显著高于该基因在相应品种其他花器官中的表达量(LSD,P<0.05)。以上结果说明CygoSTK基因在功能上有很强的保守性,主要参与春兰子房的发育。 |
| 关键词: 春兰, CygoSTK基因, MADS-box, 花发育, 实时荧光定量 |
| DOI：10.11931/guihaia.gxzw201906041 |
| 分类号:Q943.2 |
| 文章编号:1000-3142(2020)04-0518-08 |
| 基金项目:国家自然科学基金(31101202)[Supported by the National Natural Science Foundation of China(31101202)]。 |
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| Expression comparison of CygoSTK gene in Cymbidium goeringii and abnormal flower variety ‘Tian Peng Mu Dan' |
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XIA Shengying, LIU Zhixiong*
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College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China
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| Abstract: |
| In order to uncover the molecular mechanism of flower organ development regulation of Cymbidium goeringii and varieties with abnormal flower, a cDNA was cloned from the flower buds of the common C. goeringii and C. goeringii ‘Tian Peng Mu Dan' by homologous cloning method. The 849 bp D-class MADS-box gene CygoSTK(Genbank accession number is MH917912.1), which was high identity in C. goeringii and C. goeringii ‘Tian Peng Mu Dan', contained a 705 bp complete ORF, encoding 234 amino acid residues; Protein alignment and a phylogenetic tree grouped CygoSTK into the STK lineage. Structural analysis showed that CygoSTK transcription factor contained a highly conserved MADS domain(1-57)and a secondary conserved K domain(91-172); In addition, the C-terminal transcriptional activation region of CygoSTK contained two highly conserved motifs: AGI motif and AGⅡ motif. Furthermore, the relative expression of CygoSTK gene in different floral organs of C. goeringii and C. goeringii ‘Tian Peng Mu Dan' was detected by qPCR. Our data suggested that CygoSTK expression was the highest in the ovary of common C. goeringii and C. goeringii ‘Tian Peng Mu Dan', which was significantly higher than that of the other floral organs(LSD,P<0.05). Our results indicate that the function of CygoSTK gene is highly conserved and CygoSTK is mainly involved in regulating the ovary development of C. goeringii. |
| Key words: Cymbidium goeringii, CygoSTK gene, MADS-box, ploral development, real-time quantitative PCR |
