罗汉果雌雄株同工酶性别鉴定研究

李惠敏<sup>1</sup>; 黄夕洋<sup>1; 2</sup>; 高成伟<sup>1</sup>; 秦新民<sup>1*</sup>

引用本文:	李惠敏, 黄夕洋, 高成伟, 秦新民.罗汉果雌雄株同工酶性别鉴定研究[J].广西植物,2007,(5):792-795.[点击复制]
	LI Hui-Min, HUANG Xi-Yang, GAO Cheng-Wei, QIN Xin-Min.Sex identification of Siraidia grosvenorii by isozyme analysis[J].Guihaia,2007,(5):792-795.[点击复制]

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罗汉果雌雄株同工酶性别鉴定研究
李惠敏¹, 黄夕洋^1,2, 高成伟¹, 秦新民^1*
1.广西师范大学生命科学学院, 广西桂林 541004;2. 广西壮族自治区中国科学院广西植物研究所, 广西桂林 541006

摘要:

采用电泳技术结合同工酶染色,分析了罗汉果雌雄株叶片的过氧化物酶同工酶、酯酶同工酶、超氧化物歧化酶同工酶、多酚氧化酶同工酶和过氧化氢酶同工酶。结果表明:罗汉果雌雄叶片在同工酶谱上,存在着与性别性状相关的酶带; 雌雄间的差异酶带在每一种同工酶中均有一条以上,可作为罗汉果雌雄株间的性别鉴定。此外,还比较了高产、低产、不结果雌株之间同工酶的酶带和活性差别。

关键词: 罗汉果性别性状同工酶雌雄株

DOI：

分类号:Q946.5

基金项目:

Sex identification of Siraidia grosvenorii by isozyme analysis

LI Hui-Min¹, HUANG Xi-Yang^1,2, GAO Cheng-Wei¹, QIN Xin-Min^1*

1.College of Life Sciences, Guangxi Normal University, Guilin 541004, China;2. Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China

Abstract:

The peroxidase,esterase,superoxide dismutase,polyphenol oxidase and catalase isozyme of Siraidia grosvenorii were analyzed with vertical slab PAGE and specific cytochemical staining. The results showed that isozyme bands have apparent difference between the male and female plant. The number of different band of sex character was above one in each isozyme. It is concluded that the difference on isozyme bands can be used for sex identification of Siraidia grosvenorii. In addition,the isozyme bands and enzyme activity of high yield female plant,low yield female plant and acarpous female plant were compared.

Key words: Siraidia grosvenorii sex character isozyme male and female plant