铬对烟草组培苗生长和某些生理指标的影响

Effects of Cr 6+on the growth and some physiological characters of tobacco by tissue culture

关键词:  铬  烟草  组织培养  生长生理

Key words:  Cr⁶⁺  tobacco  tissue culture  growth physiology

摘要:

以烟草无菌苗为材料,在培养基中添加0、50、100、150、200、300 μmol·L^-1浓度的重金属铬离子,进行组织培养,35 d后分析植株生长和相关生理变化。结果表明,低浓度(50 μmol·L^-1)铬对烟草组培苗生长有促进作用,株高、鲜重、叶绿素含量、蛋白质含量、SOD活性呈现上升趋势; 高浓度铬处理则抑制生长,株高、鲜重、叶绿素、蛋白质含量、SOD活性都下降,相反,POD活性、MDA含量和细胞膜透性显著增加。

Abstract:

After germfree tobacco seedlings being cultivated in culture medium added with Cr⁶⁺ solution in 0,50,100,150,200,300 μmol·L^-1 consistency respectively for 35 days,the growth and certain physiological functions of the tobacco were studied. The results indicated:(1)the callus and seedlings growth was promoted at 50 μmol·L^-1 consistency,and the height,fresh weight,content of chlorophyll and protein, SOD(superoxide dismutase)activity of the seedlings increased;(2)the growth of callus and seedling was restrained distinctly under the high consistency(100～300 μmol·L^-1),and the seedling height,fresh weight,content of chlorophyll and protein,activity of SOD(superoxide dismutase)decreased;(3)on the contrary,the activity of peroxidase(POD),content of MDA and the permeability of cell membrane had earned a remarkable increase with the high concentration of Cr⁶⁺.