| 引用本文: | 梁小燕 方 宏 宁德生 陈海珊 黄永林.桂南地区苦玄参药材RP-HPLC指纹图谱研究[J].广西植物,2007,(6):948-952.[点击复制] |
| LIANG Xiao-Yan, FANG Hong, NING De-Sheng,
CHEN Hai-Shan, HUANG Yong-Lin.RP-HPLC fingerprint of Picria fel-terrae from South Guangxi[J].Guihaia,2007,(6):948-952.[点击复制] |
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| 摘要: |
| 采用反相HPLC法测定了广西梧州、龙州、苍梧、越南等多产地12批苦玄参药材指纹图谱,并对不同产地的苦玄参指纹图谱进行比较。色谱条件为:Luna C18(4.6×250 mm,5 μm)色谱柱,乙腈-水梯度洗脱,流速1.0 mL/min,检测波长254 nm,柱温25℃。结果12批苦玄参样品指纹图谱共标定了16个分离度良好的共有峰,方法的精密度、稳定性、重复性均符合国家相关规定,可作为控制苦玄参药材质量的定性标准。 |
| 关键词: 苦玄参 HPLC 指纹图谱 质量控制 |
| DOI： |
| 分类号:Q946 |
| 基金项目: |
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| RP-HPLC fingerprint of Picria fel-terrae from South Guangxi |
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LIANG Xiao-Yan, FANG Hong, NING De-Sheng,
CHEN Hai-Shan, HUANG Yong-Lin
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Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China
Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China
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| Abstract: |
| The RP-HPLC assay was used to establish the fingerprint of Picria fel-terrae from South Guangxi,and the HPLC chromatogram of different origins of Picria fel-terrae were compared. The chromatography conditions were as follows:Luna C18 column(4.6×250 mm,5 μm),a mixture of acetonitrile and water as mobile phase in gradient mode,flow rate was 1.0 mL/min,detective wavelength at 254 nm,column temperature 25℃. The fingerprints of P.fel-terrae with 16 common peaks were determined. The RSD of precision and reproducibility lay within 5%. According to this method,the established fingerprint can be used for the identification and quality control of P.fel-terrae. |
| Key words: Picria fel-terrae HPLC fingerprint quality control |
