洋桔梗体细胞胚性愈伤组织诱导过程中超氧化物歧化酶和酯酶变化

Changes of superoxide dismutase and estrase in processes of embryogenic calli induction of Eustoma grandiflorum

关键词:  洋桔梗  胚性愈伤组织  非胚性愈伤组织  超氧化物歧化酶  酯酶  同工酶

Key words:  Eustoma grandiflorum  embryogenic callus  nonembryogeneic callus  superoxide dismutase  estrase  isoenzyme

摘要:

通过转移洋桔梗非胚性愈伤组织到含有1.0 mg/L 2,4-D的MS培养基(ecIM)上诱导了洋桔梗胚性愈伤组织形成,而非胚性愈伤组织在含1.0 mg/L 2,4-D和0.5 mg/L KT的MS培养基(necSM)上继代培养。本研究比较分析了洋桔梗愈伤组织在ecIM和necSM上的超氧化物歧化酶(SOD)活性及其同工酶酶谱、酯酶同工酶酶谱随着培养天数的变化。实验结果表明在ecIM和necSM上培养的洋桔梗愈伤组织的超氧化物歧化酶(SOD)活性在培养早期较低,然后随着培养天数增加而升高,维持在较高水平上,但SOD活性变化无明显规律性; 另一方面,SOD同工酶在第4天后出现一新的同工酶谱带; 此外,在ecIM和necSM上培养洋桔梗愈伤组织的酯酶(EST)同工酶在培养至第16～20天期间呈现显著缺失。

Abstract:

Embryogenic calli induction of Eustoma grandiflorum were carried out when non-embryogenic calli on necSM(MS medium containing 1.0mg/L 2,4-D and 0.5mg/L KT)were transferred onto ecIM(MS medium containing 1.0mg/L 2,4-D). Changes of superoxide dismutase(SOD)activities and isoenzymes and estrase(EST)isoenzymes in calli of E.grandiflorum cultured on ecIM and necSM for different days of cultures have been achieved. Experimental results have been shown that SOD activities in calli of E.grandiflorum on ecIM and necSM were low in early culture times and raised along with increasing days of cultures and maintained a higher level without an evident regularity for the changes of SOD activities,and for SOD isoenzymes there was also a new band of SOD isoenzyme until 4 days of culture; Estrase(EST)isozyme in calluses of E.grandiflorum cultured on ecIM and necSM had an distinct absence in the periods from 16 days to 20 days.