火百合花器的鳞茎诱导和增殖培养研究

Study on inducion and multiplication culture ofbulblet from some parts of flower in Lilium

关键词:  火百合  花器  鳞茎诱导培养  增殖培养

Key words:  Lilium  flower organ  bulblet inducing culture  multiplication culture

摘要:

采用4.5～6.5 cm长、花苞青色未开放的火百合花蕾作材料,切取其子房、花柱、花丝为外植体进行鳞茎诱导,结果表明:花丝的鳞茎诱导率最高,其次是花柱,子房的诱导率最低。以子房和花柱为外植体诱导出的鳞茎个体大小不一(1～7 mm),以花丝为外植体诱导出的鳞茎则普遍偏小,但较整齐一致(3～4 mm); 小鳞茎诱导的最佳6-BA浓度是1.0 mg/L。研究了基本培养基、6-BA浓度、NAA浓度对小鳞茎增殖的效应及不同蔗糖浓度对小鳞茎增重的影响,结果表明:小鳞茎增殖的最佳培养基是:MS+6-BA2.0 mg/L+NAA0.2 mg/L,4%蔗糖对鳞茎增重效果最好。

Abstract:

The some parts in flower such as ovary,style and filament as explants were cultured for inducing bulblet in Lilium. The results indicated that the highest bulblet inducing rate was gotten from filament while the lowest from ovary,there were bigger difference of the bulblets from ovary and style,while there were ordinary smaller but uniform bulblet from filament,there was best inducing effect for bulblet when the media added with 1.0 mg/L 6-BA among all concentration. The uniform bulblets were studied for multiplication culture with different media,different concentration. of 6-BA and NAA,and for enlarging culture with different concentration. of sugar,the results indicated that,MS medium+2.0 mg/L 6-BA+0.2 mg/L NAA was the best one for bulblet multiplication,there was obvious effect of bulblet multiplication culture when the media added with glycine,there was the highest effect for enlarging culture of bulblets when the media added with 4%sugar,but with fewer or more concentration.