八角莲组织培养研究

Study on tissue culture of Dysosma versipellis

关键词:  八角莲  组织培养  激素

Key words:  Dysosma versipellis  tissue culture  hormone

摘要:

以八角莲种子为外植体,MS为基本培养基,通过不同的激素种类和浓度配比,对八角莲进行组织培养研究。结果表明:种子在MS+BA1.0 mg/L+IBA0.5 mg/L+GA₃4.0 mg/L培养基上容易萌芽,发芽率为72.4%; 培养基MS+BA10.0 mg/L+GA₃0.5 mg/L可诱导种子幼苗形成丛生芽; 继代繁殖在MS+BA(8.0～10.0)mg/L+GA₃2.0 mg/L与低浓度BA或无BA的培养基上进行循环培养效果较好; MS+NAA1.0 mg/L+AC0.2 g/L适宜诱导生根获得再生植株,生根率100%。带叶叶柄在MS+BA1.0 mg/L+2-ip(0.5～1.0)mg/L+NAA0.02 mg/L培养基上可诱导愈伤及根,直接形成再生植株。生根苗移栽成活率90%。

Abstract:

The seeds of Dysosma versipellis were used as explants for tissue culture in different culture media. The results obtained from the present study were as follows:the seed germination rate could reach 72.4% on MS+BA1.0 mg/L+IBA0.5 mg/L+GA₃4.0 mg/L; the medium which was good for the cluster buds induction in seed buds was MS+BA10.0 mg/L+GA₃0.5 mg/L; the medium of proliferation should be MS+BA(8.0～10.0)mg/L+GA₃2.0 mg/L with low concentration of BA or no BA for circulation culture; the ideal medium for root formation was MS+NAA1.0 mg/L+AC0.2 mg/L,and the rooting rate could reach 100%; tender leaves with stalk could be induced for callus and roots in MS+BA1.0 mg/L+2-ip(0.5～1.0)mg/L+NAA0.02 mg/L. The survival rate of the seedlings was about 90%.