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| 引用本文: | 孙同兴, 江幸山.简便有效的叶表皮离析方法——过氧化氢-醋酸法[J].广西植物,2009,(1):44-47.[点击复制] |
| SUN Tong-Xing, JIANG Xing-Shan.H2O2-CH2COOH maceration——a simple and perfect method for leaf epidermis[J].Guihaia,2009,(1):44-47.[点击复制] |
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| 简便有效的叶表皮离析方法——过氧化氢-醋酸法 |
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孙同兴1, 江幸山2
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1.盐城师范学院 生命科学与技术学院, 江苏 盐城 224002;2.华南农业大学 生命科学学院, 广州 510642
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| 摘要: |
| 在长期叶表皮制片过程中发现,过氧化氢-醋酸离析法简便实用,对蕨类植物小型叶和大型叶、裸子植物鳞形叶、钻形叶、针形叶、条形或带形叶及被子植物宽大的叶片均能适用。尤其重要的是,过氧化氢-醋酸离析法不会破坏叶表皮细胞的结构,有利于精确观察叶表皮形态特征,是一种比较理想的叶表皮离析方法。 |
| 关键词: 叶表皮 离析方法 过氧化氢-醋酸 |
| DOI： |
| 分类号:Q944.5 |
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| H2O2-CH2COOH maceration——a simple and perfect method for leaf epidermis |
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SUN Tong-Xing1, JIANG Xing-Shan2
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1.School of Life Science and Technology, Yancheng Teachers College, Yancheng 224002, China;2.College of Life Science, South China Agricultural University, Guangzhou 510642, China
1.School of Life Science and Technology, Yancheng Teachers College, Yancheng 224002, China;
2.College of Life Science, South China Agricultural University, Guangzhou 510642, China
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| Abstract: |
| The characteristics of H2O2-CH2COOH maceration for the preparation of leaf epidermis was reviewed. The maceration in equal volumes of acetic acid and 30% hydrogen peroxide didn't destroy the structures of epidermal cells and was suitable for the leaf epidermis of pteridophyte,gymnosperms and angiosperms. |
| Key words: leaf epidermis maceration hydrogen peroxide and acetic acid |
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