匙羹藤组织培养条件优化研究

苏 钛<sup>1; 2</sup>; 黄宁珍<sup>1*</sup>; 付传明<sup>1</sup>

引用本文:	苏钛, 黄宁珍, 付传明.匙羹藤组织培养条件优化研究[J].广西植物,2009,(1):87-91.[点击复制]
	SU Tai, HUANG Ning-Zhen, FU Chuan-Ming.Optimization in vitro culture conditions for Gymnema sylvestre[J].Guihaia,2009,(1):87-91.[点击复制]

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匙羹藤组织培养条件优化研究
苏钛^1,2, 黄宁珍^1*, 付传明¹
1. 广西壮族自治区中国科学院广西植物研究所, 广西桂林 541006;2.广西师范大学生命科学学院, 广西桂林 541004

摘要:

以匙羹藤无菌种子苗为外植体,通过预备实验确定基本培养基后,采用完全实验和正交设计相结合的方法,研究植物生长调节剂两因素配合和多因素组合(NAA、TDZ、2-ip、IAA、6-BA和KT)对匙羹藤芽体诱导及继代培养的影响。结果显示,匙羹藤组织培养最适的基本培养基为N68; N68+NAA 0.05 mg/L+2-ip0.4 mg/L+TDZ 0.001 mg/L利于芽体增殖,可用于继代培养; N68+NAA 0.05 mg/L+TDZ 0.005 mg/L促进植株长根,可用于生根培养。

关键词: 匙羹藤组织培养完全实验正交设计

DOI：

分类号:Q943.1

基金项目:

Optimization in vitro culture conditions for Gymnema sylvestre

SU Tai^1,2, HUANG Ning-Zhen^1*, FU Chuan-Ming¹

1. 1.Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, China;2.College of Life Sciences, Guangxi Normal University, Guilin 541004, China

Abstract:

The optimum medium for tissue culture of Gymnema sylvestre were studied by using germfree tub-seedling as explants. The effects of plant growth regulator(NAA,TDZ,2-ip,IAA,6-BA and KT)on the seedlings proliferation were studied by complete experiment and orthogonal design. The results showed that the optimum basic medium for G.sylvestre was N68; N68+NAA 0.05 mg/L+2-ip 0.4 mg/L+TDZ 0.001 mg/L was the best for bud proliferation,and can be used in subculture; N68+NAA 0.05 mg/L+TDZ 0.005 mg/L promoted rooting,can be used as rooting medium.

Key words: Gymnema sylvestre tissue culture complete experiment orthogonal design