Na2CO3胁迫下青海星星草种子萌发过程中淀粉酶活性等的变化

Change of diastase activity of Puccinellia tenuiflora cv.Qinghai seeds under sodium carbonate stress

关键词:  青海星星草  Na₂CO₃胁迫  淀粉酶  可溶性糖  呼吸强度

Key words:  P.tenuiflora cv.Qinghai  sodium carbonate stress  diastase  soluble carbohydrate  respiration intensity

摘要:

对Na₂CO₃胁迫下青海星星草种子萌发过程中淀粉酶活性、可溶性糖含量、呼吸强度的变化进行了研究。结果表明在Na₂CO₃胁迫下,种子萌发过程中淀粉酶活性、可溶性糖含量、呼吸强度与空白对照相比均下降,当Na₂CO₃浓度大于5.00%时,青海星星草种子在播种后至测量结束时,淀粉酶活性、可溶性糖含量、呼吸强度几乎都没有变化,三者与Na₂CO₃胁迫浓度的负相关关系均极其显著。 在播种后的1～8 d可溶性糖含量的变化与淀粉酶活性的变化基本趋于一致,两者呈显著的正相关关系。第8天以后,可溶性糖含量开始降低,而淀粉酶活性继续升高,说明可溶性糖已被利用、转化或合成新物质,Na₂CO₃胁迫下可溶性糖含量减少主要是因为淀粉酶活性受到了抑制。水解酶活性降低、储藏物质不能动员导致其不能水解、呼吸代谢受抑制是Na₂CO₃胁迫下青海星星草种子萌发受抑制的主要原因。

Abstract:

Changes of diastase activity, soluble carbohydrate content and respiratory intensity of germinating Puccinellia tenuiflora cv.Qinghai seeds under sodium carbonate stress were studied. The results were as follows:diastase activity,soluble carbohydrate content,respiration intensity had a positive correlation with germinating process in salt free condition. When the sodium carbonate concentration was more than 5.00%,changes of diastase activity,soluble carbohydrate content and respiratory intensity of germinating P.tenuiflora cv.Qinghai seeds nearly did not happen,but it had a significant negative correlation with each other. Until the eighth day from being bred,changes of soluble carbohydrate content was basic the same to changes of diastase activity and had a significant negative correlation with each other. But after the eighth day,soluble carbohydrate content begin to decrease and diastase activity continue to raise,it showed that soluble carbohydrate had been used, transferred or composted to new material. Why soluble carbohydrate content decreased under sodium carbonate stress was that diastase activity had been restrained. The decrease of hydrolytic enzyme activity and inhibition of the using of storing material and the inhibition of respiration were the main reasons the P.tenuiflora cv.Qinghai seeds could not germinate under sodium carbonate stress.