宁夏枸杞繁育系统初步研究

Preliminary study on the breeding system of Lycium barbarum

关键词:  宁夏枸杞  繁育系统  花粉活力  柱头可授性  套袋实验

Key words:  Lycium barbarum  breeding system  pollen vitality  stigma receptivity  bagging experiment

摘要:

运用TTC法、联苯胺-过氧化氢法、P/O比、OCI和套袋实验,对宁夏枸杞种内各参试材料花粉活力、柱头可授性及繁育系统进行了研究。结果表明:宁夏枸杞花粉数量/胚珠比大于4 000,杂交指数介于3～4,结合人工授粉和套袋实验结果可以确定在参试的13个品种(系)中麻叶系材料(宁杞1号、2号、大麻叶、小麻叶)表现为部分自交亲和; 而其它材料(白花、白条、宁杞3号、尖头圆果、圆头圆果、蒙杞1号、扁果、0701、0616)表现为自交不亲和,同株异花、异株异花几乎不坐果,自然条件下只有通过与其他材料混植的途经来实现结实。由于自交亲和水平极低,宁夏枸杞的繁育系统应属于专性异交(除麻叶系材料)。

Abstract:

The pollen viability,stigma receptivity and breeding system of Lycium barbarum were studied by using TTC,benzidine-hydrogen peroxide,pollen-ovule ratio,out-crossing index and bagging experiment. The results showed that:the ratio of pollen quantity and ovule of L.barbarum is more than 4 000,and hybrid index is between 3 and 4. The combination of artificial pollination and bagging experiments determined that among these 13 participants,Maye(Ningqi 1,Ningqi 2,Damaye,Xiaomaye)performanced partly as self-compatible,while other materials(Baihua,Baitiao,Ningqi 3,Jiantouyuanguo,Yuantouyuanguo,Mengqi 1,Bianguo,0701 and 0616 )showed self-incompatible,flower with different strains,different strains of different flower hardly setting,and under natural conditions only through mixing with other materials can they achieve replanting seed. Because of the extremely low level self-compatibility,the breeding system of L.barbarum should belong to obligate xenogamy(except the Department of Maye).