黄连抗氧化活性研究

袁王俊<sup>1; 2</sup>; 李彩芳<sup>1; 2</sup>; 丁秋瑾<sup>1</sup>; 康文艺<sup>1; 2*</sup>

引用本文:	袁王俊, 李彩芳, 丁秋瑾, 康文艺.黄连抗氧化活性研究[J].广西植物,2009,(5):694-697.[点击复制]
	YUAN Wang-Jun, LI Cai-Fang, DING Qiu-Jin, KANG Wen-Yi.Studies on the antioxidant activity of Coptis chinensis[J].Guihaia,2009,(5):694-697.[点击复制]

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黄连抗氧化活性研究
袁王俊^1,2, 李彩芳^1,2, 丁秋瑾¹, 康文艺^1,2*
1.河南大学天然药物研究所, 河南开封 475004;2.河南省天然药物与免疫工程重点实验室, 河南开封 475004

摘要:

利用DPPH、FRAP和ABTS三种抗氧化活性分析方法对黄连植物不同部位的有机溶剂提取部分进行抗氧化评价,将所测定结果与Trolox进行比较,发现黄连植物不同部位抗氧化活性不同。其中,黄连须根的抗氧化活性最高; 同一部位中,乙酸乙酯和甲醇提取物抗氧化活性一般要高于石油醚提取物。在三种方法中,黄连不同部位的提取物清除自由基的能力均随浓度增大而增大; 三种方法之间有很好的相关性,以FRAP法与DPPH法相关性最好(r=0.9261,P<0.01)。

关键词: 黄连抗氧化活性 DPPH FRAP ABTS

DOI：

分类号:Q946

基金项目:

Studies on the antioxidant activity of Coptis chinensis

YUAN Wang-Jun^1,2, LI Cai-Fang^1,2, DING Qiu-Jin¹, KANG Wen-Yi^1,2*

1.Institute of Natural Products, Henan University, Kaifeng 475004, China;2.Key Lab of Natural Drug &3.Immune Engineering of Henan Province, Kaifeng 475004, China

Abstract:

Antioxidant activities of different extracts from different parts of Coptis chinensis were evaluated by the DPPH assay,FRAP assay and ABTS assay,and the results were compared to Trolox. The results showed that different parts had different activity,and fibrous root had the highest antioxidant activity; and different polar solvent extracts of the same part also demonstrated different antioxidant activity,ethyl acetate and methanol extracts generally had higher antioxidant activity than the petroleum ether extract. The antioxidant activity of the extracts from different parts of C.chinensis increased when the concentration of the extracts increased; there were good correlation among the three methods,and the values obtained by FRAP assay were correlated best with those obtained by DPPH assay(r=0.9261,P<0.01).

Key words: Coptis chinensis antioxidant activity DPPH FRAP ABTS