松茸组织分离物的rDNA-ITS序列鉴定

张灵<sup>1; 2</sup>; 赵琪<sup>1</sup>; 陈严平<sup>1</sup>; 孟珍贵<sup>1</sup>; 李荣春<sup>1*</sup>

引用本文:	张灵,赵琪,陈严平,孟珍贵,李荣春.松茸组织分离物的rDNA-ITS序列鉴定[J].广西植物,2010,(2):181-184.[点击复制]
	ZHANGLing,ZHAOQi,CHENYan-Ping,MENGZhen-Gui,LIRong-Chun.Identification of cultures isolated from fruit body of Tricholoma matsutake by rDNA-ITS sequence analysis[J].Guihaia,2010,(2):181-184.[点击复制]

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松茸组织分离物的rDNA-ITS序列鉴定
张灵^1,2,赵琪¹,陈严平¹,孟珍贵¹,李荣春^1*
1.云南农业大学食用菌研究所, 昆明 650201;2.云南农业大学基础与信息工程学院, 昆明 650201

摘要:

以采自云南丽江的松茸子实体为材料,进行组织分离后,利用一对ITS引物（ITS1 ITS4）对子实体（SR176B,SR172B）和分离物（SR176H,SR172H）进行了PCR扩增、琼脂糖凝胶电泳分析,得到了700 bp左右的扩增条带,进一步对ITS序列进行同源性检索比对,结果表明SR176H与SR176B,SR172H与SR172B序列同源性均为100%,鉴定出该分离物就是松茸的纯培养物。

关键词: 松茸子实体分离物 ITS序列

DOI：

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Identification of cultures isolated from fruit body of Tricholoma matsutake by rDNA-ITS sequence analysis

ZHANGLing^1,2,ZHAOQi¹,CHENYan-Ping¹,MENGZhen-Gui¹,LIRong-Chun^1*

1.Insititution of Edible Fungi, Yunnan Agricultural University, Kunming 650201, China;2.College of Science and Information Engineering, Yunnan Agricultural University, Kunming 650201, China

Abstract:

A pair of general primer(ITS1 ITS4)was used to test in the amplification of the internal transcribed spacer(ITS)region of the chromosomal DNA of fruit body of Tricholoma matsutake of Lijiang and its isolates. The results analyzed by Agarose gel electrophoresis showed that the primers of ITS1 and ITS4 amplified about 700bp fragments for all samples. The ITS fragments of T.matsutake fruit body and its corresponding isolates were sequenced and aligned.Their sequences of SR176H and SR176B showed a 100% homogeneity,the same result also appeared between SR172H and SR172B.Based on these facts,SR176H and SR172H were confirmed to be pure cultures of T.matsutake.

Key words: Tricholoma matsutake fruit body mycelia rDNA-ITS