增强UV-B辐射和He-Ne激光对-小麦原生质体微管骨架的影响

Influence on microtubule in wheat mesophyll cell exposed to enhanced ultraviolet B radiation and He-Ne laser irradiation

关键词:  微管骨架  免疫荧光定位  小麦  He-Ne激光  UV-B辐射

Key words:  microtubule  immunofluorescence localization  wheat  He Ne laser  UV B radiation

摘要:

以小麦叶片原生质体为材料,采用间接免疫荧光定位法标记其微管系统,并利用激光共聚焦扫描显微系统进行观察。研究了低剂量He-Ne激光（5mW•mm-2）、增强UV B辐射（10.08kJ•m-2•d-1）及二者的复合处理对小麦幼苗叶肉细胞中微管骨架的影响。结果表明,增强UV-B辐射后,小麦叶片细胞中微管骨架发生解聚,呈短棒状或点状分布,微管束弥散且荧光强度减弱;而增强UV-B辐射后再施以He-Ne激光处理,小麦叶肉细胞微管骨架有部分断裂,但较单独UV-B处理组的损伤程度轻,说明低剂量的He-Ne激光可以部分修复增强UV B辐射对微管骨架的损伤,且对微管的聚合有促进作用。

Abstract:

In this paper,the protoplasts from fresh leaves of wheat seedlings was acted as material,which was exposed to enhanced UV-B radiation(10.08 kJ•m-2•d-1),He Ne laser irradiation(5 mW•mm-2 )and the combined treatments of UV B and He Ne laser. Effects of different treatments on microtubule(MT)cytoskeleton,which was labeled with indirect immunofluorescence localization,was studied through confocal laser scanning microscope(CLSM). The results showed that microtubule of protoplast in wheat seedlings was depolymerized significantly to stick and spot under the condition of enhanced UV-B radiation,the MT bundles was trend to dispersivity and decreasing of fluorescence intensity. However,the depolymerization of MT was decreased by He-Ne laser irradiation after enhanced UV-B radiation. Therefore,the damage of microtubule in wheat seedlings induced by enhanced UV-B radiation that can be repaired partly by He-Ne laser irradiation.