鼎湖山紫背天葵组织培养及植株再生

Tissue culture and plant regeneration in Begonia fimbristipula

关键词:  紫背天葵  愈伤组织  不定芽  再生植株

Key words:  Begonia fimbristipula  callus  adventitious buds  plant regeneration

摘要:

选用紫背天葵无菌苗叶片为外植体，建立了离体培养体系，并探讨其愈伤组织生长和不定芽诱导的适宜培养条件。通过研究得出：愈伤组织诱导的最佳培养基是MS+2，4 D 1.00 mg/L+6 BA 1.0 mg/L+LH 200 mg/L+CH 200 mg/L+YE 200 mg/L，愈伤组织诱导率为96.88%。不定芽诱导培养基为MS+6 BA 1.0 mg/L+LH 200 mg/L+CH 200 mg/L+YE 200 mg/L，不定芽诱导率为77.3%。不定芽转至1/2MS培养基中均可100%生根并长成完整植株，小苗移栽成活率达到90%。

Abstract:

An efficient and rapid regeneration protocol was developed using leaves from geminating seedlings of Begonia fimbristipula. MS medium containing 1.00 mg/L 2，4 D，1.0 mg/L 6 BA，200 mg/L LH，200 mg/L CH and YE 200 mg/L was most effective in inducing calli. The frequency of callus induction was above 96.88%. Optimal medium for inducing adventitious buds is MS+1.0 mg/L 6 BA+200 mg/L LH+200 mg/L CH+YE 200 mg/L，and the highest frequency reached 77.3%. The proper culture medium of rooting was 1/2MS and the rooting rate was 100%. The survival rate of the plantlet was up to 90% after being transplanted into soil.